国槐转雪花莲凝集素基因及抗蚜性  

Transformation of the Insecticidal Agglutinin Gene(gna) from Snowdrop (Galanthus nivalis) into Sophora japonica and Resistance of the Transgenic Plants to Aphids

作　　者：张晓英 甘敬[2] 尹伟伦[3] 朱祯[4] 王华芳[3] 

机构地区：[1]北京市琅山苗圃,北京100039 [2]北京市园林绿化局,北京100029 [3]北京林业大学生物科学与技术学院,北京100083 [4]中国科学院遗传与发育生物学研究所,北京100101

出　　处：《林业科学》2010年第2期51-55,F0003,共6页Scientia Silvae Sinicae

基　　金：国家高技术研究发展计划(863计划)资助项目(2001AA244041)

摘　　要：通过农杆菌介导法将雪花莲外源凝集素基因(gna)导入国槐叶片,获得转基因再生植株,经卡那霉素抗性筛选,PCR和Southern blot检测证实,gna基因已经整合进入国槐基因组中。凝血活性检测表明,大部分转基因植株表现出一定的凝血活性,对照未转化植株的凝血活性很低。室内离体叶片虫试试验进一步证明,转基因植株较非转基因植株有一定的抗蚜虫能力。Snowdrop （Galanthus nivalis） agglutinin （GNA） is toxic to sap sucking injurious insects of Homopteran. In this study,gna gene was successfully transformed into Sophora japonica by using the Agrobacterium tumefaciens. Transgenic plants have been obtained. Kanamycin resistant test,PCR and Southern blot bioassays confirmed that gna gene was integrated into Sophora japonica genome DNA. The test about expression of gna inhibitory activity indicated that almost every transgenic plant exhibited more or less stronger ability to agglomerate red-blood cell of sheep than the non-transformed plants. Some transgenic plants were observed to have stronger resistance to aphids than the control plants in the laboratory test.

关 键 词：国槐 雪花莲凝集素 槐蚜 根癌土壤农杆菌 基因转化 虫口密度 

分 类 号：S718.46[农业科学—林学] Q943.2[生物学—植物学]