人新基因EOLA1生物信息学分析  

Bioinformatics analysis of human novel gene EOLA1

作　　者：刘月明[1] 刘战立[2] 刘漪沦[1] 许雪峰[1] 张萍[1] 马兵[1] 

机构地区：[1]成都医学院第一附属医院烧伤整形科,四川成都610500 [2]成都医学院第一附属医院骨科,四川成都610500

出　　处：《成都医学院学报》2010年第1期1-7,共7页Journal of Chengdu Medical College

基　　金：国家自然科学基金项目(30672715)

摘　　要：目的预测人类新基因EOLA1的生物学功能。方法基于EOLA1全长cDNA序列,应用生物信息方法,从序列比对、染色体定位、基因结构分析、编码蛋白理化性质分析、蛋白质位点和序列模式预测等几个方面对EOLA1进行分析。结果EOLA1编码的蛋白EOLA1经网上同源性比对没有发现与之高度同源的人类已知蛋白;EOLA1与鼠111002L19蛋白高度同源,到目前为止,对鼠111002L19蛋白功能也不清楚;应用辐射杂交细胞系(RH)作图系统发现EOLA1定位于Xq27.4;对其二级结构进行预测发现在EOLA1蛋白分子中存在酪氨酸激酶Ⅱ和蛋白激酶C磷酸化位点以及1个螺旋-转角-螺旋(HTH)基序。结论人新基因EOLA1编码蛋白质的一级和二级结构特征赋予EOLA1信号转导能力,有可能作为信号分子在LPS激活人血管内皮细胞的过程中发挥作用。Objective To explore the biological function of human novel gene EOLA1. Methods Many bioinformatics methods were used to analyses and predict the function of EOLA1 based on the full-length cDNA of EOLA1. Results We didn＇t find any human protein high homologous with EOLA1 by homology comparison on line. However, EOLA1 was high homologous with rat 111002L19 protein of which the function was unclear up to now. The genomic DNA of EOLA1 contained 5 exons,spanning about 6 294 bps,and was mapped to human chromosome Xq27.4. We found thai EOLA1 secondary structure contained α-helix,β-Lamellosa and β-turn, and a hell.turn-helix（ HTH）motif by bioinformaties analysis. We scanned PROSITE of EOLA 1 on line, and found that EOLA 1 contained： （ 1 ） N-glycosylation site（ 36-39 NCTI） ; （ 2 ） Protein kinase C phosphorylation site （ 7 9 SFR, 33 35 SQR ） ; （ 3 ） Casein kinase Ⅱ phosphorylation site（100-103 TPDE）. Conclusion The information got by bioinformatics analysis indicales that EOLA1 may play an important role in process of Human vascular endothelial cell activation as a transcription factor.

关 键 词：新基因 EOLA1 生物信息学 

分 类 号：R318[医药卫生—生物医学工程]