低氧、无血清培养对人骨髓间充质干细胞增殖、凋亡的影响  

Effects of hypoxia and serum deprivation on the proliferation and apoptosis of human bone marrow derived mesenchymal stem cells

作　　者：蒋能刚[1] 胥劲[1] 邱琳[2] 唐莉[2] 朱鸿明[2] 李秀群[2] 陈晓禾[2] 

机构地区：[1]四川大学华西医院,成都610041 [2]华西医院生物治疗国家重点实验室干细胞与组织工程研究室

出　　处：《山东医药》2010年第11期1-3,共3页Shandong Medical Journal

基　　金：国家863计划项目(2007AA021902)

摘　　要：目的观察人骨髓间充质干细胞(hBM-MSCs)对低氧环境的适应能力,为其临床应用提供依据。方法体外培养hBM-MSCs,根据培养条件分为四组,常氧正常血清组培养条件为20%O2及10%FBS/HD、常氧无血清组为20%O2及0%FBS/HD,低氧正常血清组为1.5%O2及10%FBS/HD,低氧无血清组为1.5%O2及0%FBS/HD,细胞培养6、12、24、48、72、96 h后采用MTT法检测四组增殖状况;流式细胞术检测四组凋亡情况。结果在10%FBS培养下,48 h时低氧可显著促进细胞增殖;在整个观察时间内,低氧培养不会促进hBM-MSCs凋亡;同时无血清培养时细胞增殖受抑。结论在正常的血清培养条件下,hBM-MSCs对低氧有较好的耐受性,但是无血清培养不利于hBM-MSCs增殖,hBM-MSCs可作为缺氧相关组织修复的种子细胞。Objective To investigate whether human bone marrow derived mesenchymal stem cells could resist the hypoxia condition in vitro, so as to provide basis for its clinical application. Methods hBM-MSCs were cultured in vitro and divided into four groups according to the culture condition , the normoxia-normal serum group was cultured with 20% O2 and 10% FBS ＋ DMEM-HG（FBS/HD） , the normoxia-serum deprivation group was cultured with 20% O2 and 0% FBS/HD ,the hypoxianormal serum group was cultured with 1. 5% O2 and 10% FBS/HD,the hypoxiaserum deprivation group was cultured with 1.5% O2 and 0% FBS/HD. 6 , 12 , 24 , 48, 72 and 96 h after the culture ,the proliferation of BM-MSCs in the four groups were measured by MTT method; and the apoptosis of which were detected using flow cytometry. Results when cultured in 10% FBS/HD, hypoxia enhanced the proliferation ability of hBM-MSCs significantly in 48 h; during the total observing time, hypoxia did not up-regulate the apoptosis rate of hBM-MSCs; serum deprivation culture suppressed the proliferation of hBM-MSCs. Conclusions hBM-MSCs show better tolerance when cultured in normal serum, but serum deprivation culture is unfavorable to the proliferation of hBM-MSCs, hBM-MSCs can be promising seed cells for the repair of hypoxia-related tissue.

关 键 词：骨髓间充质干细胞 低氧 增殖 凋亡 

分 类 号：R551.3[医药卫生—血液循环系统疾病] R329.28[医药卫生—内科学]