HBIG-PBCA-NP对QSG、HepG及PBMC细胞增长率及毒性的影响  

Effects of HBIG-PBCA-NP on the cell growth rate and toxicity of QSG,HepG and PBMC

作　　者：彭忠田[1,2] 谭德明[2] 黄顺玲[2] 朱平安 刘菲[2] 

机构地区：[1]南华大学附属第一医院,湖南衡阳421001 [2]中南大学湘雅医院

出　　处：《山东医药》2010年第11期25-27,共3页Shandong Medical Journal

基　　金：湖南省长沙市科学技术局基金资助项目(K051155-72)

摘　　要：目的研究乙型肝炎免疫球蛋白（HBIG）聚氰基丙烯酸正丁酯纳米粒（HBIG—PBCA—NP）、PBCA—NP的体外应用安全性。方法用乳化聚合法分别制备HBIG-PBCA-NP、PBCA-NP；采用MTT比色法研究不同浓度的HBIG-PBCA-NP、PBCA—NP对外周血单核细胞（PBMC）、QSG7701细胞及HepG2.2．15细胞生长抑制作用的影响；用自动生化仪测定乳酸脱氢酶（LDH）、丙氨酸转氨酶（ALT）活性。结果按相对生长率（RGR％），HBIG—PBCA—NP、PBCA—NP对PBMC、QSG7701细胞及HepG2．2．15细胞的毒性分级分别为0—1级，LDH与ALT释放值与HBIG、空白对照相比，差异无显著性（P〉0．01）。结论PBCA—NP属无毒级载体，HBIG—PBCA—NP具有良好的细胞生物安全性。Objective To investigate the application security of hepatitis B immunoglobulin polybutylcynaoacrylate nanoparticles （HBIG-PBCA-NP） and polybutylcynaoacrylate nanoparticles （PBCA-NP） in vitro. Methods HBIG-PBCA-NP and PBCA-NP were prepared respectively by emulsion polymerization method. The effects of different concentrations of HBIG-PBCA-NP, PBCA-NP and HBIG on the growth inhibition of PBMCs, QSG7701 cells, HepG 2.2.15 cells were studied by MTT colorlmetric assay; the activity of lactate dehydrogenase （LDH） and alanine aminotransferase （ALT） were detected by automatic biochemy-instrumen. Results Judging by relative growth rate （ RGR% ）, the cytotoxicities grade of HBIG-PBCA-NP, PBCA-NP and HBIG for PBMCs, QSG7701 cells, HepG 2.2.15 cells were 0-1 grade, respectively. Compared to the controls,the LDH and ALT levels of HBIG-PBCA-NP, HBIG and PBCA-NP treatment groups showed no differences. Conclusion PBCA-NP is non-toxic-class carrier,HBIG-PBCA-NP has good bio-security in vitro.

关 键 词：乙型肝炎免疫球蛋白 乙型肝炎免疫球蛋白聚氰基丙烯酸正丁酯纳米粒 MTT比色法 外周血单个核细胞 QSG7701细胞 HEPG2.2.15细胞 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学] R512.6[医药卫生—基础医学]