X射线对体外培养乳鼠心肌细胞损伤的实验研究  

Experimental Research of the Damage of Myocardiai Cells Caused X-ray

作　　者：张三典 陆妙珍 高耀明[2] 张军宁[2] 王建平[2] 徐九宏[2] 

机构地区：[1]浙江宁波李惠利医院放疗科,浙江宁波315040 [2]苏州大学附属第一医院肿瘤放疗科,江苏苏州215006

出　　处：《中国辐射卫生》2010年第1期26-28,共3页Chinese Journal of Radiological Health

基　　金：宁波市医学科技计划基金资助项目(200501)

摘　　要：目的研究X射线对体外培养的心肌细胞活性及凋亡的影响。方法体外培养新生大鼠心肌细胞,分为对照组和放射组,放射组分别用X射线10Gy、20Gy、30Gy、40Gy单剂量照射心肌细胞,采用MTT法观察X射线对乳鼠心肌细胞活性的影响;血生化自动分析仪定量测定X射线作用后心肌细胞乳酸脱氢酶(LDH)的改变;吖啶橙/碘化丙啶(AO/PI)双染色法在激光扫描共聚焦显微镜下观察心肌细胞凋亡的形态学改变;流式细胞仪定量检测心肌细胞的凋亡率。结果①X射线剂量在10～40Gy均能抑制心肌细胞活性并成剂量依赖性;②细胞心肌酶谱检测到LDH释放量与X射线剂量相关;③流式细胞仪发现心肌细胞凋亡率与X射线照射剂量有相关性。结论本研究结果显示X射线对心肌细胞有毒性作用,可致心肌细胞损伤,表现为心肌细胞坏死和凋亡发生率增加。Objective To investigate the direct effect of X - ray radiation on activity and apop tosis of cultured rat cardiomyocytes in vitro. Methods The study was performed using primary cell cultures of cardiomyocytes isolated from hearts of now -born rats. After being cultured for 4 days in vitro, cardiomyocytes were irradiated with single dose of 10Gy,20Gy, 30Gy,40Gy of X--ray respectively, viability of cardiac muscle cells were assessed by MTT colorimetric assay, Myocardial of lactic acid dehydrogenase （LDH） was examined by blood biochemical autoanalyzer. Meanwhile, the Morphological characters of apoptosis were checked with laser - scanning confocal microscope by AO/PI staining and cardiac myocytes apoptosis rates were detected by flow cytometry. Results ①X - ray inhibited the growth of rat cardiac muscle cells in the dose - dependment or time - dependment manner in a certain range of dose. ②the Morphological characters of apoptosis induced by X - ray（40Gy） were surveyed by laser - scanning confocal microscope in cultured rat cardiac cells. ③X - ray induced apoptosis rate was detected being increased by flow cytometry. Conclusion X - ray has toxic effect, causing the damage of cardiac ceils or apoptosis

关 键 词：X射线 体外培养乳鼠心肌细胞 凋亡 

分 类 号：R811.5[医药卫生—放射医学]