川芎嗪对实验性肝纤维化大鼠TNF-α、IL-4和IFN-γ表达的影响  

Effect of ligustrazine on the expression of TNF-α、IL-4 and IFN-γin rats with liver fibrosis

作　　者：陈玮[1] 陈维雄[1] 金涌[1] 陆允敏[1] 

机构地区：[1]上海交通大学附属第六人民医院消化内科,上海200233

出　　处：《世界临床药物》2010年第3期153-156,共4页World Clinical Drug

摘　　要：目的观察川芎嗪对大鼠肝α肿瘤坏死因子(TNF-α)、白介素-4(IL-4)和γ干扰素(IFN-γ)表达的影响,进一步阐明川芎嗪抗肝纤维化的可能作用机理。方法80只大鼠随机分为正常对照组(N组)、模型对照组(M组)、川芎嗪治疗组(T组)和空白对照组(R组),采用四氯化碳造模。8周后,T组予以川芎嗪腹腔注射(一日1次80mg/kg),R组予以相同容量生理盐水腹腔注射,共8周。实验结束后所有大鼠处死,分别行苏木素-伊红(HE)染色,采用半定量计分系统(SSS)评估肝纤维化程度,荧光定量聚合酶链式反应(PCR)技术检测TNF-α、IL-4和IFN-γ在肝内的表达。结果T组大鼠肝纤维化程度明显低于R组,且差异有统计学意义(P<0.05);M组TNF-α、IL-4的表达明显高于N组,IFN-γ的表达明显低于N组;T组TNF-α、IL-4的表达明显低于M组,IFN-γ的表达则明显高于M组;R组TNF-α、IL-4及IFN-γ的表达介于M组和T组之间。结论川芎嗪用于大鼠肝纤维化有显著疗效,其机制可能与其能影响TNF-α、IL-4及IFN-γ等细胞因子在肝组织的表达有关。Objective To investigate the effect of ligustrazine on the expression of TNF-α, IL-4 and IFN-γ in rats with liver fibrosis. Methods Eighty SD rats were randomly devided into normal group （N group）, model group （M group）, test group （T group） and control group （R group）. Hepatic fibrosis was induced by subcutaneous injection of carbon tetrachloride. Ligustrazine was given 80 mg/kg once daily by intraperitoneal injection in T group, and 0.9% normal saline solution was given 80 mg/kg once daily in R group for 8 weeks. Hepatic fibrosis was assessed through HE staining and semi-quantitative scaling system （SSS）. The expression of TNF-α, IL-4 and IFN-γ were detected by fluorescence quantitative polymerase chain reaction （PCR）. Results The degree of hepatic fibrosis in T group was obviously reduced when compared with R group （P 〈0.05）. Compared with N group, the expression of TNF-α and IL-4 in M group were elevated significantly, whereas the expression of IFN-γ was decreased. Compared with M group, the expression of TNF-α and IL-4 in T group were decreased significantly, whereas the expression of IFN-γwas increased. Conclusion Ligustrazine has obvious therapeutic effect on rats with liver fibrosis. The mechanism may be correlated to interfering the disturbance of some cytokines such as TNF-α, IL-4 and IFN-γ.

关 键 词：川芎嗪 肝纤维化 Α肿瘤坏死因子 白介素-4 γ干扰素 

分 类 号：R282.71[医药卫生—中药学] R575[医药卫生—中医学]