内毒素性休克大鼠肺组织糖皮质激素受体mRNA的表达  

Glucocorlicoid receptor expression in the lung tissue in endotoxic shock rat

作　　者：张育才[1] 左文琼[1] 龚小慧[1] 滕国良[1] 张宇鸣[1] 

机构地区：[1]上海交通大学附属儿童医院急救中心上海交通大学儿科危重病研究所,上海200040

出　　处：《中华急诊医学杂志》2010年第2期136-139,共4页Chinese Journal of Emergency Medicine

基　　金：上海市卫生局科技发展基金（05041）

摘　　要：目的探讨内毒素（LPS）性休克大鼠肺组织糖皮质激素受体（GR）mRNA的表达。方法56只SD大鼠，随机（随机数字法）分LPS休克组（16只）、LPS＋血管活性肠肽（vasoactive intestinal peptide，VIP）组（16只）、LPS＋VIP＋糖皮质激素（GC）组（16只）和对照组（8只）。尾静脉注射LPS制作休克模型，尾静脉注射LPS10mg／kg后，分别于15min内注射VIP5nmol／kg或VIP5nmol／kg＋甲基强的松龙3mg／kg，对照组注射等容量生理盐水，分别于注射后6h和24h处死，留取肺标本，观察肺组织病理变化，RT-PCR检测肺组织GRmRNA的表达。结果（1）肺组织病理改变：LPS组见肺泡腔结构破坏、肺泡间隔增宽、炎性细胞浸润、出血、间质水肿、细胞器破坏，LPS＋VIP组和LPS＋VIP＋GC组病变较轻。（2）GRmRNA的表达：注射LPS后6h，LPS组和LPS＋VIP组GRmRNA表达下降，与对照组相比，差异具有统计学意义（P〈0．05），LPS组下降较LPS＋VIP组稍明显，但差异无统计学意义（P〉0．05）。24hGRmRNA表达恢复。LPS＋VIP＋GC组GRmRNA表达6h增加，24hGRmRNA表达更高（P〈O．05）。结论LPS致肺损伤时，肺组织GRmRNA的表达减少。VIP和GC可抵抗炎症反应、减轻肺损伤，其机制可能与增强GRmRNA的表达有关。Objective To investigate the effects of vasoactive intestinal peptide（VlP） on TLR 2,4 mRNA expressions in the lung tissue in endotoxic shock rat induced by LPS rat. Method Fifty six SD rats were randomly （random number） divided into 4 groups, including LPS shock group （ n = 16）, LPS ＋ VIP group （ n = 16）, I.PS ＋ VIP ＋ GC group （ n = 16）, and control group （ n = 8）. LPS shock model was made by intravenously inject- ing with [PS（ 10 mg/kg） ;rats in the LPS ＋ VIP group were intravenously injected with VIP after LPS; rats in the LPS ＋ VII）＋ GC group were injected with VIP and methylprednisolone together with LPS; rats in the control group were given with normal saline. The rats were sacrificed at 6 h, 24 h after injected. The lung tissues were collected, Pathological changes of the lungs were observed under light microscope and electron microscope. The GR mRNA expressions were assessed by RT-PCR in the lung tissues. Results （1）Lung histopathology： necrotic abscission cells increased in alveolar space, inflammatory cells infiltration and lung interstitial edema appeared in LPS shock group. However,pathological changes in LPS ＋ VIP group and LPS ＋ VIP ＋ GC group were milder than those in LPS shock group. （2）After 6 hours LPS injection, the GR mRNA expression in LPS group and LPS＋ VIP group were significantly lower as compared with those of the control group （ P 〈 0.05 ）, the GR mRNA expressions of LPS ＋ VIP ＋ GC group at 6 h and 24 h were significantly higher than those of the other groups （ P 〈 0.05）. Conclusions Expression of GR mRNA decreases in rats with LPS-induced lmlg injury. VIP ＋ GC can reduce lung in- jury, which may have correlation with up-regulation of GR mRNA expression.

关 键 词：糖皮质激素 糖皮质激素受体mRNA 血管活性肠肽 脂多糖 休克 炎症 肺损伤 鼠 

分 类 号：R686[医药卫生—骨科学]