δ阿片受体激动剂DADLE对脓毒症大鼠肾功能的影响  

Effects of δ-opioid receptor agonist DADLE on renal function in rats with sepsis

作　　者：秦连进[1] 冯文明[1] 鲍鹰[1] 费卯云[1] 王耀[1] 唐成武[1] 

机构地区：[1]湖州市第一人民医院普通外科,浙江省湖州313000

出　　处：《中华急诊医学杂志》2010年第2期140-144,共5页Chinese Journal of Emergency Medicine

摘　　要：目的探讨δ阿片受体激动剂DADLE对脓毒症大鼠肾功能的保护作用及其机制。方法Sprague-Dawley（SD）大鼠72只，随机（随机数字法）分为假手术组（sham-operatedgroup，SHAM组）、脓毒症组（sepsisgroup，SEP组）和DADLE处理组（DADLE-treatedgroup，DADLE组），每组2，4只。SHAM组打开腹腔后关闭，SEP组采用盲肠结扎穿孔（cecalhgation and puncture，CLP）法建立脓毒症模型，DADIE组在CLP后即静脉注射5mg/kgDALDE。各组在建模后2h，6h和10h各取8只，检测以下指标：①血清尿素氮（BUN）、肌酐（Cr）和陉．微球蛋白（-MG）。②血清肿瘤坏死因子α（TNF-α）和白介素6（IL-6）。③左肾组织三磷酸腺苷（ATP）含量。④右肾病理观察。多组间比较用方差分析，组间两两比较采用q检验。结果①DADLE组BUN和Cr分别在6h和10h比SEP组显著降低（P〈0．01），DADLE组β2-MG在各时间点均显著低于SEP组（P〈0．01）。②与SHAM组比较，SEP组和DADLE组在各时间点TNF-α和IL-6均显著升高，DADLE组显著低于SEP组（P〈0．01）。③与SHAM组比较，SEP组和DAD皿组肾组织的ATP在各时间点均降低（P〈0．01），DADLE组高于SEP组，三组间差异具有统计学意义（P〈0．01）。④光镜下见SEP组逐步出现肾小管上皮细胞肿胀、空泡变性、脱落和坏死，基底膜消失，DADLE组的肾小管上皮细胞脱落和坏死程度轻于SEP组，基底膜保持完整。结论DADLE可以改善脓毒症大鼠。肾功能，降低炎症因子水平，减缓肾组织ATP的下降，减轻肾小管上皮细胞的损害，具有肾保护作用。Objective To investigate the effects of δ-opioid receptor agonist DADLE on renal function of rats with sepsis and the underlying mechanism. Method A total of 72 Sprague-Dawley （SD） rats were randomly divided （random number） into three groups including sham-operated group （SHAM group）, sepsis group （SEP group） and DADLE-treated group （DADLE group）, each group had 24 rats. Sepsis model was produced by cecal hgation and puncture （CLP）. In SHAM group, the abdomen was opened and closed without any other treatment. In DADLE group, DADLE was administered at a dose of 5 mg/kg by intravenous injection after CLP. The following parameters of 8 rats in each group were measured at 2 h, 6 h and 10 h.（DThe serum levels of urea nitrogen （BUN）, creatinine （Cr） and β2 mieroglobulin （β2-MG）.（2）The serum levels of tumor necrosis factor α（TNF-α） and inter- lealdn 6 （IL-6）. OThe contents of adenosine triphosphate （ATP） in left kidneys. （4）The pathologic changes in right kidneys. Variance analysis was used in multiple comparison and q test was used to compare between two groups. Results （1）Levels of BUN and Cr in DADLE group were lower than those of SEP group at 6 h and 10 h, respectively （P 〈 0.01）, and levels of β2-MG were significantly lower than SEP group at all times （P 〈 0.01）. （2） Levels of TNF-a and IL-6 of rats both in SEP and DADLE groups were significantly higher than those SHAM group （ P 〈 0.01 ）, and both these parameters in DADLE group were significantly lower than those SEP group （ P 〈 0.01）.（3）Levels of ATP both in DADLE and SEP groups were decreased compared with SHAM group,and ATP in DADLE group were higher than that of SEP group. The differences were significant in the three groups （ P 〈 0.01）. （3）The hydropic, hyaline degeneration and necrobiosis in tubular epithehal cells and disappearance of basement menabrane could be seen step by step in SEP group, and DADLE group was better than SEP group. Condnsions De

关 键 词：Δ阿片受体激动剂 DADLE 脓毒症 盲肠结扎穿孔 肾功能 大鼠 尿素氮 肌酐 Β2-微球蛋白 三磷酸腺苷 

分 类 号：R686[医药卫生—骨科学]