大豆GmCOL8基因的克隆及表达分析  

Cloning and Expression Analysis of GmCOL8 Gene in Soybean (Glycine max L.)

作　　者：马锦花[1] 张清哲[1] 陈新建[1] 傅永福[2] 

机构地区：[1]河南农业大学生命科学学院,郑州450002 [2]中国农业科学院作物科学研究所

出　　处：《植物生理学通讯》2010年第1期17-23,共7页Plant Physiology Communications

基　　金：国家支撑计划(2007BAD59B02);转基因专项(2008ZX08009-001;2008ZX08004-005;2008ZX08010-004);"863"项目(2006AA10Z107;2006AA10A111;2007AA10Z119);"973"项目(2004CB117206)

摘　　要：从大豆品种‘垦农18’中克隆了一个CONSTANS-like基因,命名为GmCOL8。进化树分析表明它属于CONSTANS-like亚家族1的成员。mRNA表达分析显示,GmCOL8在短日下具有明显的生物钟节律性表达特性,其表达高峰出现在凌晨。GmCOL8主要在复叶中表达,表达高峰出现在开花时期。A CONSTANS-like gene, named GmCOL8, was cloned from soybean （Glycine max） cultivar ＇Kennong 18＇ in the study. The analysis of phylogenetic tree showed that GmCOL8 was a member of soybean CONSTANS-Iike subfamily group 1. The mRNA expression pattern revealed that GmCOL8 had rhythmicity of circadian clock under the condition of short day, and reached a peak at dawn. It expressed mainly in trifoliolates, and the maximum level appeared at the stage of flowering.

关 键 词：大豆 CONSTANS-like 生物钟节律 生物信息学 

分 类 号：S565.1[农业科学—作物学] Q943.2[生物学—植物学]