珠美海棠试管苗的土壤支撑生根培养和带坨移栽  

Rooting in Soil Supporting Medium and Transplanting with Lump of Malus zumi (Matsumura) Rehd Plantlet in vitro

作　　者：柴慈江[1] 卢兴霞[1] 苏卫国[1] 裴君[1] 王玉[1] 

机构地区：[1]天津农学院园艺系,天津300384

出　　处：《植物生理学通讯》2010年第1期33-36,共4页Plant Physiology Communications

基　　金：国家科技部星火计划(2008GA610015);天津市科委项目(08ZHXHNC07000)

摘　　要：以粘壤土做培养基支撑物,选用只含有0.5mg·L-1IBA和15g·L-1蔗糖的液体培养基,珠美海棠试管苗茎段生根培养的结果显示,试管苗生根率可达到100.0%,明显高于琼脂支撑培养,根长也显著提高,并且长出正常的根毛。土支撑培养生根的珠美海棠试管苗开瓶炼苗21d后带坨移入营养钵中,移栽后不喷雾、并毋需覆盖塑膜、午后空气相对湿度控制在50%～65%,其成活率达到92.2%;在开瓶炼苗21d后,黑暗中叶片气孔的关闭率从26.7%增加到88.5%。Stem sections from the Malus zumi plantlet were cultured in vitro in lipid medium with clay loam soil supporting, and the medium contained 0.5 mg.L^-1 IBA and 15 g.L^-1 sugar addition. The rooting rate was 100.0%, which was obviously higher than that of the plantlets cultured in agar supporting medium. The plantlets cultured in soil supporting medium had root hairs and the roots were obviously longer than those of the plantlets cultured in agar medium. The rooted plantlets cultured in soil supporting medium were acclimatized in opened bottles for 21 days, and then were transplanted with lump into soil. Without spraying water or covering plastic film, and under relatively low air humidity from 50% to 65% in the afternoon, 92.2% of the plantlets were survived. Observation the stomata of the M. zumi plantlets in vitro showed that the stomata closing rate of the plantlets in dark condition increased from 26.7% to 88.5% after acclimatized in opened bottle for 21 days.

关 键 词：殊美海棠 试管苗 土支撑 生根 带坨移栽 

分 类 号：S682.19[农业科学—观赏园艺]