溶析结晶纯化去乙酰真菌环氧乙酯  

Purify Deacetylmycoepoxydiene with Solventing-out Crystallization

作　　者：周显凤[1] 黄耀坚[1] 郑忠辉[1] 沈月毛[1] 

机构地区：[1]厦门大学生命科学学院,福建厦门361005

出　　处：《厦门大学学报（自然科学版）》2010年第2期242-245,共4页Journal of Xiamen University：Natural Science

基　　金：国家海洋863项目(2007AA091503)

摘　　要：为考察溶析结晶纯化去乙酰真菌环氧乙酯的效果,研究了不同的溶剂、反溶剂含量和导入方式对去乙酰真菌环氧乙酯结晶的影响.在静态结晶实验的基础上,通过设计正交实验L9(34)研究动态结晶过程中各因素对去乙酰真菌环氧乙酯收率和纯度的影响.结果表明:有效地控制反溶剂含量及其导入方式可以提高晶体的纯度和收率并影响晶体的形态.去乙酰真菌环氧乙酯动态结晶最佳条件:结晶温度为15℃,溶剂-反溶剂体积比(丙酮-水)为2∶3,反溶剂流加时间为3 h.In order to purify deacetylmycoepoxydiene（DAM）using solventing-out crystallization technique, the optimal conditions of crystallization were investigated by orthogonal design,and the recovery and purity of DAM were used as major evaluating contents. The results showed that the shapes of DAM crystal were influenced by different solvents,the recovery and purity of DAM were also influenced by the volume of anti-solvent and the way it was conducted. The optimal parameters are described as follows： crystallization temperature is 15 ℃ ,V（acetone） ： V（water）=2 ： 3,the flowing time of anti-solvent is 3 hours.

关 键 词：溶析结晶 去乙酰真菌环氧乙酯 正交实验 

分 类 号：TQ026[化学工程]