黄淮冬麦区小麦冬、春性改良及分子标记辅助选择技术初探  

Genetic Improvement of Wheat Growth Habit and Its Molecular Marker-Assisted Selection in Yellow and Huai River Reaches

作　　者：张晶[1] 吴锁伟[1] 刘秉华[1] 宋梅芳[2] 周朋[1] 郭春燕[3] 詹克慧[3] 王山荭[1] 杨丽[1] 董冬[1] 于立强 李辉利 周阳[1] 杨建平[1] 

机构地区：[1]中国农业科学院作物科学研究所/农作物基因资源与基因改良重大科学工程,北京100081 [2]山东省农业科学院蔬菜研究所,山东济南250100 [3]河南农业大学农学院,河南郑州450002 [4]河北省赵县农业科学研究所,河北石家庄051530

出　　处：《作物学报》2010年第3期385-390,共6页Acta Agronomica Sinica

基　　金：国家高科技研究发展计划(863计划)项目(2006AA10Z1F2);国家重点基础研究发展计划(973计划)项目(2009CB118300)资助

摘　　要：通过对小麦品种石麦12春化特性的遗传和分子标记研究,探索黄淮冬麦区小麦冬、春性改良途径和分子标记辅助选择技术。石麦12与冬性品种石家庄8号杂交后代F2:3株系中的春性株系、冬春性分离株系、冬性株系的分离比例符合1∶2∶1,表明石麦12具有一个显性春化基因,经已知春化基因的基因特异性标记鉴定为Vrn-D1。利用Vrn-D1的基因特异性标记对上述F2:3株系进行冬、春性鉴定的结果与表型鉴定结果一致,说明该分子标记可用于小麦冬、春性改良中对Vrn-D1的辅助选择。在高海拔、长日照地区夏播是小麦冬、春性表型鉴定的一个快速、简便途径。The vernalizatioin gene in wheat cultivar Shimai 12 （Triticum aestivum L.） was investigated to find a more efficient way for the improvement of growth habit of wheat cultivars grown in Yellow and Huai River Reaches. In the F2：3 lines derived from the cross between Shimai 12 and Shijiazhuang 8 （a winter wheat cultivar）, the ratio of the spring, segregating, and winter lines was in accordance to 1：2：1 based on chi-square test, showing that the spring growth habit of Shimai 12 was determined by single dominant gene, which was identified as Vrn-D1 by gene-specific markers. The Vrn-D1 gene-specific marker was further used to genotype the F2：3 lines, and the results were consistent with phenotyping, verifying the available utilization of the marker assisted selection for Vrn-D1 in breeding programs. Besides, summer sowing in high altitude and cool area proved to be a cost-saving and efficient method for wheat growth habit identification.

关 键 词：小麦育种 春化基因 分子标记 

分 类 号：S512.1[农业科学—作物学]