陕253γ-醇溶蛋白基因的克隆与序列分析  

Gene Cloning and Sequence Analysis of γ-Gliadin Genes from Wheat Cultivar Shaan 253

作　　者：王明霞[1] 高翔[1,2,3] 陈其皎[1,2,3] 董剑[1,2,3] 赵万春[1,2,3] 李艳亮[1] 李敏[1] 陈瑞佶[1] 庞红喜[1,2,3] 李哲清[1,2,3] 刘俊[1,2,3] 

机构地区：[1]西北农林科技大学农学院 [2]陕西省小麦工程技术研究中心 [3]陕西省小麦新品种培育工程研究中心,陕西杨凌712100

出　　处：《作物学报》2010年第3期526-532,共7页Acta Agronomica Sinica

基　　金：陕西省“13115”科技创新工程重大项目(2007ZDKG-01);现代农业产业技术体系建设专项(NYCYTX-001)资金资助

摘　　要：针对γ-醇溶蛋白基因家族成员,设计了覆盖其启动子及全长编码区的3对特异引物,从强筋小麦品种陕253中克隆了8条1000bp左右的片段(GenBank登录号为GQ871770～GQ871777)。该片段群包含典型醇溶蛋白亚基的完整编码序列,并在重复区存在丰富的插入/缺失(InDel);推导的氨基酸序列显示,8个基因均具有γ-醇溶蛋白亚基的典型结构特征,其中GQ871771为假基因,4条序列(GQ871770、GQ871772、GQ871776和GQ87177)具有9个半胱氨酸残基;启动子区序列分析表明,GQ871770、GQ871772、GQ871774和GQ871776在胚乳框存在6处SNP变异,其中两处变异发生于GCN4基序内,利用WebLogo3在线构建了储藏蛋白更具代表意义的30bp保守胚乳盒模式。进化分析证实克隆序列属于γ-醇溶蛋白基因家族成员。Eight γ-gliadin genes （GenBank accession number GQ87170 to GQ87177） were cloned from wheat （Triticum aestivum L.） cultivar Shaan 253 using three sets of specific primers, which were designed according to the known γ-gliadin gene family. All of the eight genes possess the typical structure of γ-gliadin subunits and many sequences of insent/indel in the repeat region. The deduced amino acid sequences showed that GQ871771 is a pseudogene, and nine cysteine residues existed in GQ871770, GQ871772, GQ871776, and GQ87177. Promoter sequences analysis indicated that there were six SNPs in endosperm box of GQ871770, GQ871772, GQ87174, and GQ871776, and two of them appeared in the GCN4-like motif. A model for endosperm box of storage protein with better representation was constructed using the online tool WebLogo3. Phylogenetic analysis also confirmed that the cloned sequences belong to γ-gliadin gene family.

关 键 词：普通小麦 γ-醇溶蛋白 基因克隆 序列分析 

分 类 号：S512.1[农业科学—作物学]