地方性克汀病患者外周血淋巴细胞核T_3受体研究  

Triiodothyronine Nuclear Receptor in Lymphocytes from Patients with Endemic Cretinism

作　　者：赵学勤[1] 张镜宇[1] 苏学良[1] 李桐芬 李军山[3] 

机构地区：[1]天津医科大学内分泌研究所,300070 [2]天津肿瘤医院 [3]天津市胸科医院

出　　处：《标记免疫分析与临床》1998年第4期202-206,共5页Labeled Immunoassays and Clinical Medicine

摘　　要：本文报告了利用放射配体分析方法对9例克汀病患者及17名正常人进行了外周血淋巴细胞核T3受体的研究，通过Scatchard分析确定了各自的核T3受体的亲和常数和最大结合容量，结果表明：克汀病患者与正常人的亲和常数（Ka）一致，分别为（2．13±O．69）×1010L／mol和（1．74±0．13）×1010L／mol，而最大结合容量（MBC）则有显著差别，分别为18．53±6．97fmol／100μgDNA和4．38±1．8fmol／100μgDNA（P＜0．01）。The circulating lymphocyte was chosen as the target cell to study the T3- receptor of human patients with cretinism for elucidating whether there is any difference of alteration of the T3-receptor from adult cretins as comparing with that of mormal subject. The isolated lymphocytes were incubated with 0. 5mL of Eagle's medium containing increasing concentrations of L -T3 and fixed amounts of 125I-T3 at 37℃ for 2 hours. The bound radioactivity was measured after two washes with SMT-Triton buffer solution and the binding characteristics were analyzed by Scatchard's method. In lymphocyte from the health subjects there are T3 binding sites with an apparent mean affinity constant (Ka) of (1. 74 ±0. 13 ) ×10(10) L/mol, and the estimated mean maximum binding capacity (MBC) for T3 is 4.38 ±1 .87 fmol/100μg DNA. In cretinistic patients, Ka and MBC for T3 are (2. 13±0. 69 ) ×10(10)L/mol and 18. 53±6. 97fmol/100μg DNA,respectively. The difference of MBC between normal and cretinism is very significant, and Ka is not.

关 键 词：克汀病 淋巴细胞 T3受体 

分 类 号：R581.210.3[医药卫生—内分泌]