原位反转录PCR检测慢性丙型肝炎患者外周血单个核细胞中HCV  

DETECTION OF HEPATITIS C VIRUS IN PERIPHERAL BLOOD MONONUCLEAR CELLS OF PATIENTS WITH CHRONIC HEPATITIS C BY IN SITU REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION

作　　者：蔡美顺[1] 冯百芳[1] 刘玉兰[1] 

机构地区：[1]北京医科大学人民医院肝病研究所

出　　处：《中华肝脏病杂志》1998年第4期211-212,共2页Chinese Journal of Hepatology

基　　金：国家自然科学基金

摘　　要：目的研究慢性丙型肝炎患者外周血单个核细胞（ｐｅｒｉｐｈｅｒａｌｂｌｏｏｄｍｏｎｏｎｕｃｌｅａｒｃｅｌ，ＰＢＭＣ）中丙型肝炎病毒的定位分布及复制。方法直接原位反转录－ＰＣＲ（标记引物）对２０例慢性丙型肝炎患者的ＰＢＭＣ内丙型肝炎病毒（ＨＣＶ）的分布、定位及存在形式进行研究。结果１６例患者外周血单个核细胞的胞浆内发现ＨＣＶＲＮＡ阳性信号，并有一例患者的胞浆内发现ＨＣＶＲＮＡ负链信号。结论ＨＣＶ可感染ＰＢＭＣ并在其中复制。原位ＲＴ－ＰＣＲ为进一步研究ＨＣＶ进入人体内的分布、潜伏、复制状况以及丙型肝炎治疗、药物研究奠定了基础。Objective To detect the localization, distribution, replication of HCV in PBMCs of patients with chronic hepatitis. Method Direct in situ RT-PCR with labelled primer. Results In 20 patients, 16(80%) were found positive for HCV genome within their PBMCs by in situ RT-PCR, and 1 patient was detected HCV RNA negative strand. These positive signals were localized in cytoplasma of PBMCs. Conclusion PBMCs could be an important extrahepatic site of virus replication. The destribution, localization, latent replication of HCV in bodies of patients and effect of anti-viral drug might be studied by in situ RT-PCR. HCV persisted in PBMCs can interfere with the immunologic mechanism of the patient and play a role in the pathogenic progress.

关 键 词：丙型肝炎病毒 原位反转录 PCR 外周血 

分 类 号：R512.63[医药卫生—内科学]