丙型肝炎病毒核酸定量分析的临床意义  

THE CLINICAL SIGNIFICANCE OF QUANTITATIVE POLYMERASE CHAIN REACTION FOR HCV RNA IN PATIENTS WITH HEPATITIS C

作　　者：张玲霞[1] 马洪滨[1] 李伯安[1] 郭仁峰[1] 胡瑾华[1] 乔林林 程云[1] 

机构地区：[1]解放军302医院

出　　处：《中华肝脏病杂志》1998年第4期227-228,共2页Chinese Journal of Hepatology

摘　　要：目的探讨慢性丙型肝炎患者血清丙型肝炎病毒核酸（ＨＣＶＲＮＡ）与病情及治疗的关系。方法应用美国ＡｃｕＧｅｎ公司提供的定量ＰＣＲ试剂盒，通过荧光检测仪对５０例丙型肝炎患者的血清ＨＣＶＲＮＡ作定量检测。结果５０例患者血清ＨＣＶＲＮＡ均值为（１０００５±３３１６）×１０７拷贝／Ｌ，ＨＣＶＲＮＡ水平与ＡＬＴ呈正相关（ｒ＝０６８６７，Ｐ＜００５）。有输血史的患者ＨＣＶＲＮＡ水平明显高于无输血史者。ＨＣＶＲＮＡ血清水平低者对ＩＦＮ－α反应较好。结论ＨＣＶＲＮＡ定量检测在一定程度上反映肝脏炎症活动度及肝损害程度，有助于指导抗病毒治疗。Objective To evaluate the relationship between the serum level of HCV RNA and condition of patients with chronic hepatitis C. Methods HCV RNA in serum of 50 patients with chronic hepatitis C (CHC) was determined by quantitative polymerase chain reaction(PCR). The kits were used from AcuGen Co. USA that were based on the principle of fluorescence energy transfer. Results The mean HCV RNA level in serum of 50 patients with CHC was 100 05×10 7±33 16×10 7 The serum level of HCV RNA was correlated with ALT level. Antiviral effect of IFN-α on a lower lever HCV RNA was better than that on a higher one. Conclusion The results show that quantitative PCR is of great value in evaluating the degree of inflammative activity in liver and the efficacy of antiviral therapy.

关 键 词：丙型肝炎病毒 定量分析 聚合酶链反应 病情 治疗 

分 类 号：R512.63[医药卫生—内科学]