维甲酸对成纤维细胞作用的体外观察  

The effect of retinoic acid on fibroblast in vitro culture

作　　者：宋维铭[1,2] 孙广慈[1,2] 管正玉[1,2] 徐承熊[1,2] 张国荣[1,2] 曹蕊[1,2] 申智慧[1,2] 冯越蹇[1,2] 

机构地区：[1]中国医学科学院中国协和医科大学整形外科医院 [2]中国医学科学院药物研究所

出　　处：《中华医学美容杂志》1998年第4期190-194,共5页

基　　金：国家卫生部基金

摘　　要：目的探讨维甲酸治疗瘢痕的作用机理及其用于临床的可能性。方法采用成纤维细胞体外培养技术，并将３ＨＴｄＲ（胸腺嘧啶核苷）掺入成纤维细胞ＤＮＡ中，研究维甲酸对体外培养成纤维细胞生长增殖，ＤＮＡ合成及超微结构的影响。结果维甲酸对成纤维细胞生长增殖，ＤＮＡ合成有不同程度的抑制作用，呈剂量效应关系；扫描电镜和透射电镜观察显示，维甲酸对成纤维细胞合成胶原有抑制作用。结论维甲酸治疗瘢痕的作用机理可能与抑制成纤维细胞生长增殖。Objective To investigate the possible mechanism of retinoic acid used for the treatment of scar, and clarifying its possibilities in the application of treatment of scar. Methods The effect of retinoic acid on growth proliferation, DNA synthesis and ultrastructure of fibroblasts was studied by means of the techniques of the fibroblast in vitro culture and the 3HTdR incorporation into DNA of fibroblasts. Results The results demonstrated that retinoic acid inhibits the growth proliferation and DNA synthesis of fibroblasts in vitro culture and a dose dependent relationship between growth proliferation, DNA synethesis of fibroblasts and retinoic acid concentration was observed. Electron microscopic observateion (SEM and TEM) showed that fibrous substances on the cell surface and outside the cell had decreased. All the results indicated that retinoic acid inhibits collagen synthesis of fibroblasts. Conclusion The mechanism of retinoic acid used for the treatment of scar have a bearing on the inhibitation of growth proliferation, interruption of DNA synthesis and interference of collagen synthesis of the fibroblasts.

关 键 词：维甲酸 成纤维细胞 瘢痕 治疗 

分 类 号：R619.605[医药卫生—外科学]