辛伐他汀对人脐静脉内皮细胞ABCA1表达的影响  

The Effect of simvastatin on the expression of ABCA1 in Human umbilical vein endothelial cells

作　　者：刘芃[1] 李公信[1] 刘映峰[1] 吕俊豪[1] 缪绯[1] 徐琳[2] 赵欢[1] 

机构地区：[1]南方医科大学珠江医院心血管内科,广州510282 [2]广州军区广州总医院心血管内科,广州510010

出　　处：《广东医学》2010年第4期416-418,共3页Guangdong Medical Journal

基　　金：广东省科技计划资助项目(编号:2007B031507001)

摘　　要：目的研究辛伐他汀对人脐静脉内皮细胞的三磷酸腺苷结合盒转运体A1(ABCA1)表达的影响。方法体外培养人脐静脉内皮细胞,并分别与BSA(对照组)及1、10μmol/L的辛伐他汀组温育24h。采用逆转录聚合酶链反应、流式细胞技术分别检测人脐静脉内皮细胞的ABCA1的mRNA及蛋白表达水平。结果随着给药浓度的增加,人脐静脉内皮细胞的ABCA1表达逐步升高,与对照组比较差异有统计学意义(P<0.05)。结论辛伐他汀的调脂途径可能与其上调人脐静脉内皮细胞的ABCA1表达有关。Objective To study the effect of simvastatin on the expression of ATP-binding cassette transporter A1（ABCAl）in human umbilical vein endothelial cells（HUVECs）.Methods The HUVECs were cultured in vitro and incubated with simvastatin at different concentrations（0μmol/L,1μmol/L,10μmol/L）for 24h,and ABCA1 expression levels were determined by RT-PCR and flow cytometry.Results Simvastatin increased ABCA1 expression in HUVEC in a concentration-dependent manner（P0.05）.Conclusion Simvastatin increases ABCA1 expression in HUVECs,which is related to its lipid lowering properties.

关 键 词：三磷酸腺苷结合盒转运体A1 胆固醇流出 辛伐他汀 动脉粥样硬化 

分 类 号：R285.5[医药卫生—中药学]