固相萃取/高效液相色谱法对肝癌细胞中多西紫杉醇的测定  

Solid Phase Extracted/HPLC Method for Determination of Docetaxel in Hepatocellular Cancer Cells

作　　者：方志娥[1] 曲斐[1] 尚京川[1] 

机构地区：[1]重庆医科大学药学院,重庆400016

出　　处：《分析测试学报》2010年第3期276-279,共4页Journal of Instrumental Analysis

摘　　要：建立了固相萃取／高效液相色谱法测定肝癌细胞中多西紫杉醇浓度的方法。细胞样品经三氯乙酸沉淀蛋白，Bond—elutC18固相萃取柱提取，采用AgilentTC—C18（5μm，4．6mm×150mm）色谱柱分析，以乙腈-0．02mol／L醋酸铵缓冲液（体积比50：50，醋酸调至pH5．0）为流动相，流速为1mL／min，检测波长为230nm，进样体积为20止。多西紫杉醇的质量浓度在0．05～2．25mg／L范围内线性关系良好，相关系数r=0．9993，检出限为0．03mg／L，提取回收率为93％～95％，其日内、日间精密度（n=5）不大于6．9％，且稳定性良好。方法简便、快速、灵敏度高、干扰小，可用于生物样品中低浓度多西紫杉醇的测定。To establish a high performance liquid chromatographic （HPLC） method for determination of docetaxel in hepatocellular cancer cells. Docetaxel was extracted from hepatocellular cancer cells by a Bond-elut C18 solid phase extraction（SPE） column, and separated on a Agilent TC-C18 （5 μm,4.6mm×150mm） column by using mobile phase consisted of ammonium acetate buffer（0. 02 mol/ L, pH 5.0） - acetonitrile（50 ： 50, by volume） with a flow rate of 1 mL/min at detection wavelength of 230 nm. The result indicated that the calibration of docetaxel was linear in the range of 0. 05 - 2. 25 mg/L with correlation coefficient of 0. 999 3. The detection limit for docetaxel was 0. 03 mg/L. The extraction recoveries of docetaxel form forin cells sample ranged from 93% to 95%. The within-day RSDs（n = 5） and the between-day RSDs （ n = 5 ） were both not more than 6.9%. The method was convenient, accurate and sensitive, and could be applied in the determination of low level of docetaxel in biological samples.

关 键 词：高效液相色谱 固相萃取 多西紫杉醇 肝癌细胞 

分 类 号：O657.72[理学—分析化学] O622.3[理学—化学]