反相高效液相色谱法测定鼻炎滴剂中绿原酸和黄芩苷的含量  

Determination of Chlorogenic Acid and Baicalin in Coryza Dripping Solution by Reversed-phase High Performance Liquid Chromatography

作　　者：刘伟娜[1] 高洁[1] 张雪[1] 禇文静[1] 黄喜茹[1] 侯海妮[1] 

机构地区：[1]河北医科大学药学院,河北石家庄050017

出　　处：《时珍国医国药》2010年第3期640-641,共2页Lishizhen Medicine and Materia Medica Research

基　　金：河北省卫生厅资助项目(No.04102);河北省中医药管理局资助项目(No.05057)

摘　　要：目的建立测定鼻炎滴剂中绿原酸和黄芩苷含量的方法。方法采用RP-HPLC法,色谱柱为Diamonsil^TMC18（250mm×4.6 mm,5μm）柱,以甲醇-水-磷酸（52∶48∶0.1）为流动相,检测波长320nm,流速为1.0 ml·min^-1。结果绿原酸在4.3034.40μg·ml^-1范围内线性关系良好,回归方程为：A=127.3C-4.396,r=0.999 9,平均回收率102.1%,RSD=0.94%;黄芩苷在15.52108.64 g·ml^-1范围内线性关系良好,回归方程为：A=38.15C＋55.1,r=0.999 7,平均回收率100.8%,RSD=1.4%。结论该法灵敏、简便、准确,可用于鼻炎滴剂的质量控制。Objective To establish a method for determination of chlorogenic acid and baicalin in coryza dripping solution.Methods RP-HPLC method was used.The column was DiamonsilTM C18（250 mm×4.6 mm,5 μm）,the mobile phase was methanol-water-phosphoric acid（52∶48∶0.1）,the flow rate was 1.0 ml·min^-1,the detection wavelength was 320 nm.Results The linearity of chlorogenic acid was good in the range of 4.30～34.40μg·ml-1,regress equation was A=127.3C-4.396,r=0.9999,and average recovery rate was 102.1%,RSD=0.94%;the linearity of baicalin was good in the range of 15.52～108.64μg·ml-^1,regress equation was A=38.15C ＋55.1,r=0.999 7,average recovery rate was 100.8%,RSD=1.4%.Conclusion The method is sensitive,simple,accurate,and can be used for the quality control of coryza dripping solution.

关 键 词：鼻炎滴剂 绿原酸 黄芩苷 反相高效液相色谱 

分 类 号：R284.2[医药卫生—中药学]