植物源农药杀虫与抑制几丁质酶活性的测定  

Assay of the Insecticidal Activity and Chitinase Inhibitory Activity of Botanical Pesticides

作　　者：周桂[1] 孙果宋 黄科林 林润国 张桂华[1] 邓光辉[1] 

机构地区：[1]广西民族大学化学与生态工程学院,广西南宁530006 [2]广西化工研究院,广西南宁530004

出　　处：《时珍国医国药》2010年第3期732-733,共2页Lishizhen Medicine and Materia Medica Research

基　　金：广西壮族自治区自然科学基金(No.0229012;No.0640038);广西教育厅项目(No.200707MS034)

摘　　要：目的研究青蒿叶、桉树叶、红树植物白骨壤叶乙醇提取物对大蜡螟虫的毒杀作用以及对虫体几丁质酶活性的抑制作用。方法采用室内药液喷虫法测定3种植物乙醇提取物的杀虫活性与抑制几丁质酶活性。结果青蒿、桉树、红树植物白骨壤叶乙醇提取物对大蜡螟24h的校正死亡率分别为(69.00±3.87)%,(53.47±11.63)%,(18.60±6.70)%。对大蜡螟几丁质酶的抑制率分别为(20.33±1.42)%,(20.59±1.72)%,(13.56±1.58)%。结论青蒿叶与桉树叶植物乙醇提取物的杀虫活性与抑制几丁质酶活性显著高于红树植物白骨壤叶乙醇提取物(P<0.05)。Objective To study the effects of southernwood leaves,eucalyptus leaves and mangrove avicennia marina leaves ethanol extracts on the insecticidal activity and chitinase inhibitory activity against Galleria mellonella.Methods The insecticidal activity and chitinase inhibitory activity of the three plant ethanol extracts were tested for indoor contact toxicity by using sprinkling method.Results The results showed that after 24 h treatment,the corrected death ratios of southernwood,eucalyptus and mangrove avicennia marina leaves ethanol extract were（69.00±3.87）%,（53.47±11.63） %,（18.60±6.70） % and the inhibition ratios of chitinase were（20.33±1.42）%,（20.59±1.72）%,（13.56±1.58）%,respectively.Conclusion The insecticidal activity and chitinase inhibitory activity of southernwood and eucalyptus leaves ethanol extract were higher than mangrove avicennia marina leaves ethanol extract markedly.

关 键 词：植物源农药 大蜡螟 杀虫活性 几丁质酶 

分 类 号：S433[农业科学—农业昆虫与害虫防治]