苦豆子的组织培养及植株再生的研究  

Tissue culture and plant regeneration of Sophora alopecuroides

作　　者：曹有龙[1] 李晓莺[1] 罗青[1] 贝盏临[1] 

机构地区：[1]宁夏农林科学院枸杞工程技术研究中心,银川750002

出　　处：《广西植物》2010年第1期102-105,共4页Guihaia

基　　金：宁夏回族自治区科技攻关项目(2001-015-04)~~

摘　　要：用4种诱导培养基P1(MS+2,4-D0.5mg/L)、P2(MS+6-BA0.1mg/L+NAA0.5mg/L)、P3(MS+6-BA0.5mg/L+NAA0.5mg/L)、P4(MS+NAA1.0mg/L+KT0.5mg/L),3种分化培养基(MS+6-BA1mg/L;MS+6-BA0.5mg/L;MS+6-BA1mg/L+NAA0.2mg/L)和4种生根培养基(MS;MS+IBA1mg/L;MS+IBA1mg/L+IAA0.5mg/L;1/2MS+IAA0.2mg/L)对苦豆子愈伤组织进行诱导和植株再生,研究影响苦豆子组织培养的因素,结果表明愈伤组织的诱导频率主要依靠激素的种类和浓度,培养基中加入0.2～2mg/L的2,4-D有利于苦豆子愈伤组织生长,但使褐化发生时间提前;培养基中加入活性炭对苦豆子愈伤组织褐化有明显的抑制作用;加入IAA对苦豆子根的分化是必需的。In order to study the factors that influence the tissue culture of Sophora alopecuroikes,four callus-inducing media,i.e.P1（MS＋2,4-D 0.5 mg/L）,P2（MS＋6-BA 0.1 mg/L＋NAA 0.5 mg/L）,P3（MS＋6-BA 0.5 mg/L＋NAA 0.5 mg/L）,P4（MS＋NAA 1.0 mg/L＋KT 0.5 mg/L）,three different media（MS＋6-BA 1 mg/L;MS＋6-BA 0.5 mg/L;MS＋6-BA 1 mg/L＋NAA 0.2 mg/L）,and four root-inducing media（MS;MS＋IBA 1 mg/L;MS＋IBA 1 mg/L＋IAA 0.5 mg/L;1/2 MS＋IAA 0.2 mg/L）were applied. The results showed that the induction frequency of calluses highly depended on the varieties and concentration of hormone in media. 2,4-D in media,with concentration ranging from 0.2-2 mg/L,was favorable to the growth of calluses of S.alopecuroikes,accompanying earlier browning occurrence. It was found that adding active charcoal into medium could efficiently inhibit the callus browning. In addition,IAA seemed an essential ingredient in medium to the root differentiation of S.alopecuroikes.

关 键 词：苦豆子 组织培养 植株再生 

分 类 号：Q943.1[生物学—植物学]