硫酸氧化同步荧光法测定猪肉中的螺旋霉素残留  

Determination of Spiramycin Residue in Pork by Synchronous Fluorimetry with Sulphuric Acid Oxidation

作　　者：张艳[1,2] 陈冠华[1] 郝庆红[1] 陈志敏[1,2] 郭云霞[1] 

机构地区：[1]河北农业大学生命科学学院,河北保定071001 [2]河北农业大学食品科技学院,河北保定071001

出　　处：《河北大学学报（自然科学版）》2010年第1期64-68,共5页Journal of Hebei University(Natural Science Edition)

基　　金：河北省自然科学基金资助项目(202281)

摘　　要：螺旋霉素被浓硫酸氧化后由非荧光物质转化为荧光物质,采用发射波长和激发波长相差25 nm的波长间隔扫描其400～600 nm内的同步荧光光谱,可于494 nm获得最强荧光发射.基于此,建立了一种测定螺旋霉素残留的新方法.在最优条件下,线性动态范围为0.02～4 mg/L;检出限为0.33μg/kg;样品的加标回收率100.31%～107.78%.该方法已用于猪肉中螺旋霉素残留的测定.Nonfluorescent spiramycin can be oxidized into fluorescent substance with concentrated sulfuric acid. When the synchronous fluorescence spectra are scanned from 400 nm to 600 nm at the emission and excition wavelength difference of 25 nm, the maximum fluorescence intensity can be measured at 494 nm. A new assay is developed to determine spiramycin residue based on the principle. Under the optimum condition, linear dynamic range is 0.02-4 mg/L; detection limit is 0. 33μg/kg; the recoveries of spiked sample is between 100.31%and 107.78%. The method has been successfully applied to the determination of spiramycin residue in pork.

关 键 词：螺旋霉素 硫酸 氧化 同步荧光 

分 类 号：O657.31[理学—分析化学]