光学生物传感器用于快速检测卡介苗活菌数研究  

Rapid Detection of the Viability of BCG Vaccine Using Optical Biosensor

作　　者：刘晓红[1,2] 赵爱华[3] 罗金平[1] 高从军[4] 田青[1,2] 王国治[3] 蔡新霞[1,2] 

机构地区：[1]中国科学院电子学研究所传感技术联合国家重点实验室,北京100190 [2]中国科学院研究生院,北京100190 [3]中国药品生物制品检定所,北京100050 [4]中国兵器工业集团北京北方医院,北京100089

出　　处：《传感技术学报》2010年第1期1-4,共4页Chinese Journal of Sensors and Actuators

基　　金：国家"863"高技术研究发展计划资助(2009AA03Z411);中国科学院知识创新项目资助(KGCX2-YW-111-2);国家科技重大专项资助课题(2008ZX10003003-02);中国科学院优秀奖资助项目

摘　　要：基于三磷酸腺苷(ATP)光学反应原理,结合实验室自制的光学生物传感器,构建了一种快速检测卡介苗(BCG)活菌数的方法。选用加热裂解法提取BCG活菌内ATP,对BCG疫苗进行了检测。结果表明,BCG活菌浓度与相对发光强度(RLU)线性相关,相关系数为0.9908(P<0.01),其ATP含量与文献报道的结果处于同一数量级(10-18mol/CFU)。检测方法的相对标准偏差(RSD)为6.17%。检测样品仅需30μL,检测时间小于30min。与国外商业化检测系统的测试结果线性相关,相关系数为0.9676(P<0.01)。这种方法简便快速,在BCG疫苗及其他活菌疫苗质量控制方面具有广泛的应用前景。Based on the principle of ATP bioluminescence reaction,a rapid method was developed for detecting the viability of BCG（Bacillus Calmette Guerin）vaccine which combined homemade optical biosensor.ATP（adenosine triphosphate）extraction was performed in boiling Tris-EDTA buffer in the experiments.In this study,a high correlation between the viable count of BCG and Relative luminescence intensity（RLU）was obtained with correlation coefficient of 0.9908（P〈0.01）according to the linearity of ATP standard,and a low Relative Standard Deviation（RSD）of 6.17%.The ATP content of BCG was 10^-18 mol/CFU,consistent with the results in papers.The sample volume was 30 μL and the whole test for a sample was completed within 30 min.There was also a high correlation between homemade optical biosensor and the commercial system with correlation coefficient of 0.9676（P〈0.01）.With the characters of easy operation and fast responses,this method can be applied widely in routine quality control to estimate viable count of BCG and other vaccines.

关 键 词：光学生物传感器 活菌浓度 生物荧光法 BCG 

分 类 号：TP212.2[自动化与计算机技术—检测技术与自动化装置]