ISSR分子标记在芦柑提纯复壮研究中的应用  

ISSR molecular marker and its application to the research on purification and rejuvenation of citrus

作　　者：王江波[1] 潘东明[1] 钟凤林[1] 郭志雄[1] 陈跃飞 尤有利 

机构地区：[1]福建农林大学园艺学院/福建农林大学园艺产品贮运保鲜研究所,福建福州350002 [2]福建永春农业局,福建泉州362600

出　　处：《亚热带农业研究》2010年第1期52-55,共4页Subtropical Agriculture Research

基　　金：国家科技支撑计划项目(2007BAD07B01)

摘　　要：采用ISSR(Inter-simple Sequence Repeat)分子标记技术,从随机引物中筛选出能稳定扩增的14条引物,利用2条引物对供试的10个芦柑样品进行PCR扩增,获得了与母本完全一致的条带,从而可以确定鉴定样本为珠心苗。In this experiment, by inter-simple sequence repeat （ISSR） molecular marker technology, 14 primers were selected from random primers, and 2 primers were successfully used for PCR amplifyling the DNA of 10 citrus specimens. The same bands as their parents＇ were achieved, consequently, it was proved that the identificated specimens were nucellar seeding.

关 键 词：ISSR分子标记 芦柑 提纯复壮 

分 类 号：S666.1[农业科学—果树学]