高效液相色谱法测定律复康胶囊中人参皂苷Re和Rg1的含量  

Analysis of panax ginseng C.A.Mey in Lvfukang Capsules

作　　者：陈舒茵[1] 申洪超[1] 黎瑞汝[1] 韩丽华[2] 王振涛[2] 梁生旺[1] 

机构地区：[1]广东药学院中药学院,广州510006 [2]河南省中医院

出　　处：《中国现代药物应用》2010年第1期1-2,共2页Chinese Journal of Modern Drug Application

基　　金：河南省科技创新人才计划项目(项目编号:0621002700)

摘　　要：目的建立律复康胶囊中红参人参皂苷Rg1与Re的含量测定方法。方法采用HPLC法本品红参中人参皂苷Rg1、人参皂苷Re。Diamonsil C18柱色谱柱,流动相为乙腈-0.05%磷酸水（21.5：79.5）,检测波长203nm。结果人参皂苷Rg1、Re在范围内呈良好线性,分别为2.33-20.95μg（r=0.9994n=5）,0.972-8.748μg（r=0.9992,n=5）,平均回收率分别为100.2%（RSD=1.9%）、97.1%（RSD=0.8%）。结论HPLC方法简便、准确,精密度高、重现性好,适用于含人参皂苷Rg1、Re成分产品含量的测定。Objective To analyze the content of panaxsaponin Rg1 panaxsaponin Re in Lvfukang Capsules.Methods HPLC was used to analyze the contents o f panaxsaponin Rg1 panaxsaponin Re in Lvfukang Capsules.The Diamonsil C18 column（5 μm,250×4.6 mm）was used.The mobile phase consisted of acetonitrile-0.05% orthophosphoric water（21.5：79.5）.The UV detector wavelength was 203 nm.Results The linear ranges of ginsenosideRgl,ginsenoside Re were2.33-20.95 μg（r=0.9994 n=5）、0.972-8.748 μg（r=0.9992,n=5）with average recoveries of 100.28%（RSD=1.89%）,97.12%（RSD=0.76%）,respectively.Conclusion The method is simple,reliable and stable,which could be used for the quality control of Lvfukang Capsules.

关 键 词：律复康胶囊人参皂苷Rg1 人参皂苷RE 高效液相色谱法 

分 类 号：R286.0[医药卫生—中药学]