随机扩增多态性DNA技术在绿蝇分类鉴定中的应用  

Application of RAPD Technique in Lucilia Classification and Indetification

作　　者：吴薇 王芙蓉 徐颖 夏德峰 

机构地区：[1]北仑检验检疫局,浙江宁波315800

出　　处：《安徽农业科学》2010年第7期3354-3355,共2页Journal of Anhui Agricultural Sciences

摘　　要：采用RAPD技术对丝光绿蝇、铜绿蝇、亮绿蝇、紫绿蝇进行区分,并对反应体系和程序进行一系列优化,从中选出可以区分4种绿蝇的引物和特异性条带。RAPD技术为绿蝇的种类鉴定提供了一种全新的鉴定方法,成为形态学分类的一种有效的补充。RAPD technique was adopted to identify four species,such as Lucilia sericata,Lucilia cuprina,Lucilia illustris and Lucilia porphyrina.Reaction system and program were optimized,and primers and specific bands were selected to distinguish the above four species.RAPD technique became an effective supplement to morphological method as a new identification method.

关 键 词：RAPD 绿蝇 分类鉴定 应用 

分 类 号：Q965[生物学—昆虫学]