依地福新抑制Jurkat细胞生长机制的研究  

Effect of Edelfosine on prolifeation of Jurkat cells

作　　者：谷彬[1] 张辉[2] 喻泽兰[1] 

机构地区：[1]湘南学院基础医学课部药理学教研室 [2]湘南学院化学与生命科学系药学教研室,湖南郴州423000

出　　处：《湘南学院学报（医学版）》2009年第3期13-15,共3页Journal of Xiangnan University（Medical Sciences）

摘　　要：目的观察依地福新对体外培养的Jurkat细胞的生长抑制作用,并进一步探讨其作用机制。方法应用MTT法检测细胞增殖活性;流式细胞仪检测细胞周期分布;Annexin V-FITC染色法检测细胞凋亡率。结果不同浓度依地福新处理Jurkat细胞24～96 h后,细胞增殖显著受到抑制,并呈现浓度及时间依赖性;1.0μmol/L、5.0μmol/L、10.0μmol/L依地福新处理72 h后,Jurkat细胞G_0/G_1期细胞数量显著增加,S期细胞数量显著降低(P<0.01);各浓度组细胞凋亡率均显著增加(P<0.01)。结论依地福新对Jurkat细胞具有生长抑制作用,其机制与阻滞细胞周期及诱导凋亡有关。Objective To investigate the effect of edelfosine on the proliferation of jurkat cells and its mechanism. Methods Jurkat cells cultured in RPMI 1640 were treated with 0.5,1.0,5.0,10.0 μmol/L of edelfosine. MTT assay, flow cytometry, and Annexin V - F1TC staining were performed to determine the cell proliferation activity, cell cycle, and apoptoric rate. Results A fiter jurkat cells were treated with different concertrations of edelfosine for 24 to 96 hours, the cell proliferation activity was inhibited in a dose - and time - dependent manner, The nrmber of cells in G0/G1 phase singnificatly increased and the number of cells in S phase singnificantly increased in cells treated with different concentrations of edelfosine （all P 〈 0.01）. Conclusion Edelfosine could inhibit the growth of jurkat cells by blocking cell cycle and inducing apoptosis.

关 键 词：依地福新 JURKAT细胞 凋亡 

分 类 号：R979.1[医药卫生—药品]