利用报告基因试验检测饮用水源水的内分泌干扰活性  

Endocrine -Disrupting Effects of Drinking Source Water with the Reporter Gene Assay

作　　者：王晓祎[1] 张哲海 胡伟[1] 孙宏[3] 王心如[3] 于红霞[1] 

机构地区：[1]南京大学环境学院污染控制与资源化研究国家重点实验室,南京210093 [2]南京市环境监测站,南京210013 [3]南京医科大学公共卫生学院,南京210029

出　　处：《生态毒理学报》2009年第5期669-674,共6页Asian Journal of Ecotoxicology

基　　金：江苏省科技计划项目(No.BS2007050);江苏省环境监测科研基金资助项目(No.0710);江苏省普通高校研究生科研创新计划资助项目(No.CX07B_170z);南京大学研究生科研创新基金资助项目(No.2007CL11)

摘　　要：基于非洲猴肾CV-1细胞受体转录激活试验,研究了饮用水源水内分泌干扰活性的检测方法,并测定了南方某城市某水厂水源水中有机提取物的拟雌激素活性和拟/抗甲状腺激素活性.结果表明,CV-1细胞受体转录激活试验是一种筛选和定量分析具有拟雌激素受体活性和拟/抗甲状腺受体活性的内分泌干扰物的快速、有效的方法.结合固相萃取等前处理技术,有效检测了南方某城市某水厂水源水弱极性和强极性有机提取物的内分泌干扰活性.此水源水弱极性组分和强极性组分,皆可以显著诱导雌激素活性,诱导倍数为对照组的6~7倍,且弱极性组分的拟雌激素活性要略强于极性组分.弱极性组分和强极性组分在不同浓缩倍数下皆不会显著诱导甲状腺激素活性,但当与5nmol·L-1T3共同作用于CV-1细胞时,弱极性组分在25~100倍浓缩时,会产生显著的拟甲状腺激素活性,且表现为与5nmol·L-1T3协同作用;在25~100倍浓缩的强极性组分暴露下,无显著的拮抗甲状腺激素活性,但当样品浓度上升至200倍浓缩暴露时,表现为显著拮抗甲状腺激素活性.对应的化学分析表明,该水厂水源水中含有有机氯农药类化合物及其代谢物（0.09~0.33ng·L-1）、多氯联苯类化合物（0.06~0.1ng·L-1）、多环芳烃类化合物（0.6~19.0ng·L-1）和辛基酚（49ng·L-1）等内分泌干扰物,很好地印证了样品内分泌干扰活性的来源.In this study, we developed and applied ER -mediated and TR-mediated reporter gene assay in evaluating the endocrine -disrupting activity of surface water containing mixed organic compounds, base on African kidney cell （CV -1 cell）. The assays were used to determine the estrogenic activity and（anti）thyroid hormone activity of organic extracts from a waterworks located in the south of China. The results proved that the reporter gene assay technique is an efficient and rapid method for screening and quantitatively analyzing the endocrine disrupters which could induce the estrogenic and （anti）thyroid hormone activity. The results also indicated that the nonpolar fraction and polar fraction of drinking source water from the waterworks could induce significant estrogenic activities. The relative potency was 6~7 fold of control and the estrogenic activity induced by nonpolar fraction was stronger than that induced by polar fraction. In series dilutions, both nonpolar fraction and polar fraction could not induce the significant thyroid hormone activity. However, exposed with 5nmol·L -1 T3, the nonpolar fraction could induce significant thyroid hormone activity, combined with 5nmol·L -1 T3. The polar fraction, only in 200 -fold concentrates, could suppress thyroid hormone activity induced by 5nmol·L -1 T3 significantly. The results of chemical analysis indicated that organochlorine pesticides and their metabolite （ranged from 0.09ng·L -1 to 0.33ng·L -1）, polychlorinated biphenyls （ranged from 0.06ng·L -1 to 0.1ng·L -1）and polycyclic aromatic hydrocarbons（ranged from 0.6ng·L-1 to 19.0ng·L-1）were detectable in the nonpolar fraction and octylphenol（49ng·L-1）in polar fraction, which had been proved their endocrine-disrupting activities in publications.

关 键 词：水源水 有机提取物 雌激素活性 甲状腺激素活性 

分 类 号：X832[环境科学与工程—环境工程] X171.5