丝素蛋白载体负载神经生长因子在周围神经损伤修复中应用的实验研究  

Repairing peripheral nerve defect by silk protein loading nerve growth factor

作　　者：汤锋[1] 刘爽 王伟[3] 

机构地区：[1]中国人民解放军第205医院烧伤整形科,锦州121001 [2]锦州市妇婴医院产科,121001 [3]锦州市中心医院骨科,121001

出　　处：《中华损伤与修复杂志（电子版）》2009年第5期16-18,共3页Chinese Journal of Injury Repair and Wound Healing(Electronic Edition)

摘　　要：目的研究丝素蛋白载体负载神经生长因子(NGF)在动物体内修复周围神经缺损的效果。方法将24只SD大鼠随机分为4组:A组、B组、C组、D组,每组6只,其中A组以丝素蛋白载体承载NGF,B组行自体神经移植,C组应用生理盐水+NGF,D组使用空白对照。术后12周测量各组修复神经动作电位幅度和传导速度,行苏木精-伊红(HE)染色观察再生神经纤维,透射电镜观察再生神经轴突和髓鞘的超微结构。应用统计学方法分析相关实验资料。结果12周后A组神经传导速度、动作电位幅度同C、D组相比差异有统计学意义(P<0.05),同B组相比差异没有统计学意义(P>0.05);HE染色可见A、B组再生神经纤维量较多、直径粗大,D组神经纤维数量相对较少,直径细小,C组神经纤维数量最少,有大量无髓神经纤维生成;透射电镜观察A组、B组神经纤维髓鞘厚度较厚,轴突结构完整,D组神经纤维髓鞘厚度较薄,C组神经纤维髓鞘厚度最细小,轴突结构不完整。结论丝素蛋白生物相容性好,作为载体能够控释NGF,在修复周围神经缺损中发挥了良好作用。Objective To research silk fibroin carrier which loads nerve growth factor （NGF）, as well as observe its repairing of peripheral nerve defect in animals. Methods Twenty- four SD rats were randomly assigned to experimental group （A） in which silk protein carried nerve growth factor and control group （B, C, D） , and after 12 weeks sciatic nerve conduction velocity and action potentials amplitude were measured, regenerated nerve fibers through the conventional hematoxylin-eosin staining, also axonal regeneration and ultrastructure of myelin were observed with transmission electron microscope. Statistical methods were used to analys the correlation experiment data. Results On the twelfth week, the nerve conduction velocity and action potential amplitude of group A were significantly different （P 〈 0.05） from group C and group D, not different compared with group B （ P 〉0.05 ） . HE staining showed that the amount of regenerated nerve fibers were more and diameter was thicker in group A and B, relatively less number of nerve fibers and smaller diameter were found in group D, the least number of nerve fibers and a large number of non-myelinated nerve fiber were found in group C. Regenerated nerve fibers in group A and B could be found thicker myelin and had completed axon structure by transmission electron microscope. Regenerative nerves was thin in sheath during group D. The regenerative nerves of group C were the smallest, and axon of which showed non-integrity. Conclusions Silk fibroin carrier bearing nerve growth factor shows biocompatibility and plays positive roles effect in peripheral nerve repairing.

关 键 词：组织工程 丝素蛋白 神经生长因子 周围神经 

分 类 号：R651.3[医药卫生—外科学]