ABO位点限制性扩增片段长度多态性的研究  

Study on amplified restriction fragment length polymorphisms of ABO locus

作　　者：李晓平[1] 杜庆新[1] 顾明波[1] 王剑[1] 车德仁[1] 朱爱民[2] 

机构地区：[1]黑龙江省公安厅四处遗传室,哈尔滨150008 [2]黑龙江省公安厅四处血清室,哈尔滨150008

出　　处：《中国法医学杂志》1998年第4期197-201,共5页Chinese Journal of Forensic Medicine

摘　　要：建立了PCR扩增、限制性酶切、8％（T）、5％（C）聚丙烯酸胺凝胶垂直电泳和银染检测ABO位点的限制性片段长度多态性的方法体系。应用Amp-RFLP技术对185名中国人（哈尔滨）ABO位点的基因频率和基因型分布进行了调查和统计分析。ABO位点特异片段长度为140～200bp，基因频率为0．2000～0．5568。6种基因型频率为0．973～0．3135，杂合度0．5838，Dp值0．7146。经H－W平衡吻合度检测，完全符合群体遗传多态分布。通过对11个家庭33名相关个体的分析，证明完全符合孟德尔遗传定律。ABO基因型检验适用于法庭科学的个体识别和亲权鉴定。An analysis method of amplification restriction fragment length polymorphism (Amp-RFLP) was established for ABO genotyping. The gene and genotype frequency distribution of ABO locus in a sample of 185 unrelated Chinese individuals in Harbin were studied. The amplified fragments were 140 ~200bp, gene frequencies 0. 2000~0. 5568, 6 genotype frequencies 0.0973 ~ 0. 3135, heterozygosity 0. 5838 and Dp value 0. 7146. The equilibrium balance test was in good agreement with H-W law. The analysis of 33 relative individuals from 11 families indicated that genes were inherited according to the Mendelian law. It is suggested that ABO genotyping is a very valuable tool for individual identifying and paternity testing in forensic science practice.

关 键 词：ABO基因型 聚合酶链反应(PCR) 限制性扩增片段长度多态性(Amp-RFLP) 

分 类 号：D919.4[医药卫生—法医学]