扩张型心肌病患者CD4^+CD25^+Foxp3^+ T细胞的检测及意义  

Significance of the change of CD4^+CD25^+ Foxp3^+ T cells in patients with dilated cardiomyopathy

作　　者：吴海[1] 廖钦晨[1] 贺慧娟[1] 陈世德[1] 伍伟锋[1] 黄伟强[1] 闭奇[1] 

机构地区：[1]广西医科大学第一附属医院心内科,南宁530021

出　　处：《临床心血管病杂志》2010年第2期90-93,共4页Journal of Clinical Cardiology

基　　金：广西自然科学基金资助(桂科自No:0640108)

摘　　要：目的:探讨扩张型心肌病(DCM)患者外周血CD4+CD25+Foxp3+T细胞的水平及意义。方法:采用流式细胞术检测DCM患者30例及健康对照组20例外周血CD4+CD25+T细胞和CD4+CD25+Foxp3+T细胞的比例。结果:DCM患者外周血CD4+CD25+T细胞占CD4+T细胞的比例为(8.53±1.64)%,显著低于健康对照组的(11.4±2.17)%,P<0.01;DCM患者CD4+CD25+Foxp3+T细胞占CD4+T细胞比例为(0.99±0.54)%,显著低于健康对照组的(1.55±0.55)%,P<0.01;且DCM患者心功能越差,CD4+CD25+Foxp3+T细胞占CD4+T细胞的比例越低。结论:DCM患者调节性T细胞比例的减少,可能打破了自身免疫耐受,发生了针对心肌抗原的自身免疫反应,参与了DCM的发病。Objective：To investigate the expression and significance of CD4^＋ CD25^＋ Foxp3^＋ T cells in peripheral blood of patients with dilated cardiomyopathy （DCM）. Method：The frequencies of CD4^＋ CD25^＋ T cells and CD4^＋CD25^＋Foxp3^＋ T cells were detected in 30 patients with DCM （DCM group） and 20 healthy donors （control group） by flow cytometry. Result：The frequency of CD4^＋ CD25^＋ T cells （[8.53±1.64]%） was found to be significantly lower in patients with DCM than those of the control group（[11.4±2.17]%） ,P〈0.01. The frequency of CD4^＋ CD25^＋ Foxp3^＋ T cells （[0.99±0.54]%）was found to be significantly lower in patients with DCM than that of the control group （[1.55±0.55]% ）,P〈0.01. And the frequency of CD4^＋CD25^＋Foxp3^＋ T cells had negative correlation with heart function grade（（NYHA） （r=0.693,P〈0.01）.Conclusions：The frequency of regulatory T cells decrease in patients with DCM and is associated with aggravated heart function. The reduction of regulatory T cells may lead to the down-regulation of autoimmune tolerance and bring about autoimmunity cardiomyopathy.

关 键 词：心肌病 扩张型 CD4^+T细胞 CD4^+CD25^+Foxp3^+T细胞 调节性T细胞 

分 类 号：R542.2[医药卫生—心血管疾病]