白细胞介素-17在心肌缺血再灌注损伤中的作用  

Preliminary research on the effect of interleukin-17 in myocardial ischemia reperfusion injury

作　　者：夏霓[1,2] 唐婷婷[1,2] 刘英[1,2] 周素峰[1,2] 严欣欣[1,2] 朱争凤[1,2] 聂少芳[1,2] 刘娟[1,2] 张文才[1,2] 杨艳[1,2] 廖玉华[1,2] 程翔[1,2] 

机构地区：[1]华中科技大学协和医院心内科 [2]华中科技大学同济医学院心血管病研究所生物靶向治疗教育部重点实验室,武汉430022

出　　处：《临床心血管病杂志》2010年第2期130-134,共5页Journal of Clinical Cardiology

基　　金：国家自然科学基金资助(No:30600234;30871067)

摘　　要：目的:通过大鼠心肌缺血再灌注损伤(IRI)模型,分析白细胞介素-17(IL-17)在IRI中的作用。方法:用阻断大鼠冠状动脉左前降支的方法制作IRI模型。Realtime-PCR及Western blot方法动态观察IRI后不同时间点心肌组织IL-17的表达水平,流式细胞术进一步检测IL-17的来源。应用抗IL-17抗体体内干预,观察其对IRI的作用。结果:IRI后1h就有IL-17的表达,并且在24h的观察期内持续存在,未有峰值出现。流式细胞术检测结果显示心肌组织中IL-17的主要来源是CD4+T细胞。用抗IL-17的抗体体内干预后,血清肌钙蛋白T的水平降低,心肌梗死面积也明显减小。结论:IL-17参与大鼠IRI过程,中和IL-17能明显减轻心肌IRI。Objective：To investigate the effect of interleukin-17（IL-17） in myocardial ischemia reperfusion injury （IRI） in rat.Method：The myocardial ischemia reperfusion injury model was performed by temporary occlusion of left anterior descending in rats. The expression of IL-17 in myocardial tissue was detected by Real time-PCR and western blot at different times after reperfusion. The source of IL -17 was confirmed by flow cytometry. Rats were treated with anti-IL-17 antibody in vivo for determining if IL -17 was involved in myocardial IRI. Result：The expression of IL-17 was detected as early as 1 hour after reperfusion,lasted for 24 hours,and showed no peak in this period. The results of flow cytometry revealed that CD4^＋T lymphocyte was a major source of IL-17 in myocardial tissue after reperfusion. Administration of anti-IL-17 resulted in a significant decrease in serum troponin T and myocardial infarct size. Conclusion：IL-17 is involved in the pathogenesis of myocardial IRI.

关 键 词：心肌缺血再灌注损伤 白细胞介素-17 T淋巴细胞 

分 类 号：R619[医药卫生—外科学]