整合素β1对人体滋养层细胞黏附和迁移行为的影响  

Effect of Integrin β_1 on Adhesion and Migration of Human Trophoblast Cells

作　　者：刘万钱[1] 管章委[1] 邓小燕[2] 李娜[1] 

机构地区：[1]重庆大学生物工程学院,重庆400044 [2]北京航空航天大学生物与医学工程学院,北京100191

出　　处：《生物医学工程学杂志》2010年第1期67-70,108,共5页Journal of Biomedical Engineering

基　　金：国家自然科学基金资助项目(10572017)

摘　　要：在体内,滋养层细胞是通过何种方式到达子宫螺旋动脉血管并对其基础结构进行重构的机制目前尚不清楚,但这一过程涉及到了细胞在血管内黏附和迁移的行为。采用微管吸吮技术和平行平板流动腔系统对整合素β1在滋养层细胞黏附和迁移中的作用进行检测。将滋养层细胞分别培养在I型鼠尾胶原和血管内皮细胞上,进行细胞黏附力测量和剪切应力加载,实验分为整合素β1抗体阻断组、非特异性抗体阻断组和正常对照组。结果显示:整合素β1抗体阻断组可显著降低细胞的黏附力和抵抗流体剪切应力引起位移的能力,而非特异性抗体组的结果与对照组差异不显著;内皮细胞可显著增强滋养层细胞的黏附力和抵抗流体剪切应力引起位移的能力。结果表明,整合素β1参与了滋养层细胞与血管内皮细胞间的黏附并调控了流体剪切应力作用下滋养层细胞的迁移行为。Although the mechanism by which migratory trophoblasts reach the spiral arteries is currently obscure,yet the process has been noted to involve the attachment,adhesion and migration of trophoblasts on the blood vessel walls.To test this,micropipette and flow chamber were used to measure quantitatively the adhesion forces and migration of early gestation human trophoblast cells（TCs） cultured on the glass slides coated with type I rat collagen or cultured with human umbilical vein endothelial cells（HUVECs）.The results showed that the interdiction of integrin β1 interaction remarkably reduced the adhesion forces of TCs to type I rat collagen or endothelial cells,and remarkably resisted the displacement of TCs induced by shear stress.By contact between TCs and endothelial cells,the TCs＇ adhesion force and TCs＇ resistance to shear stress were significantly enhanced.The results indicated that the contacts of TCs with endothelial cells enhanced the adhesion forces of human TCs,and regulated the migration of human TCs by shear stress.

关 键 词：滋养层细胞 微管 剪切力 整合素Β1 

分 类 号：R714.1[医药卫生—妇产科学]