人脐带间充质干细胞与蚕丝素多孔支架的体外复合培养  

Features of human umbilical cord mesenchymal stem cells cultured on silk fibroin porous scaffolds in vitro

作　　者：刘毅[1] 肖宏涛[1] 

机构地区：[1]兰州军区兰州总医院烧伤整形科,兰州730050

出　　处：《中华医学美学美容杂志》2010年第1期45-48,共4页Chinese Journal of Medical Aesthetics and Cosmetology

基　　金：基金项目：国家自然科学基金资助项目（编号：30872689）;全军“十一五”医学科学技术研究面上项目（编号：06MA079）;甘肃省自然科学基金资助项目（编号：3ZS061-A25-099）

摘　　要：目的观察蚕丝素多孔支架对人脐带间充质干细胞（human umbilical cord mesenchymal stem cells，hUCMSCs）吸附作用及支架对hUCMSCs形态、功能及活性的影响，为脂肪组织工程支架选择提供实验依据。方法将hUCMSCs制成细胞悬液接种在蚕丝素多孔支架，荧光倒置相差显微镜、扫描电镜和四甲基偶氮唑蓝（MTT）法观察hUCMSCs的吸附和生长情况。结果培养1～2d后可见hUCMSCs与蚕丝素多孔支架充分附着。培养5～7d细胞生长增殖十分活跃，10d左右时，蚕丝素多孔支架孔中hUCMSCs成片状融合。荧光倒置相差显微镜和扫描电镜见细胞与支架黏附良好并有大量基质分泌，且活性指标与正常培养的hUCMSCs比较差异无统计学意义（P〉0．05）。结论蚕丝素多孔支架对hUCMSCs具有良好的吸附作用，并能维持其正常形态、功能及活性，蚕丝素多孔支架是hUCMSCs三维立体培养时的良好天然支架。Objective To observe the attachment, shape, function and activity of human umbilical cord mesenchymal stem cells cultured on silk fibroin porous scaffolds in vitro, and to provide experimental foundation for the choice of scaffold in the study of adipoeyte tissue engineering. Methods These silk fibroin porous scaffolds were subsequently seeded with hUCMSCs and cultured in vitro. The growth and function of hUCMSCs were observed and measured with fluorescence inverted microscopy,scanning electronic microscopy and MTT methed. Results hUCMSCs were fixed on silk fibroin porous scaffolds 1 or 2 days later, and muhiplicative growth was observed on the 5th to 7th day. After about 10 days,the microholes of the scaffolds were overlayed by hUCMSCs. Scanning electronic microscopy and fluorescence inverted microscopy showed that cells adhered to scafold well and there was a lot of extra cellular matrix surrounding cells. Conclusion Silk fibroin porous scaffolds are ideal for attachment, growth, function maintenance and activity of hUCMSCs, and the scaffolds can be used as natural scaffolds for hUCMSCs in 3D culture.

关 键 词：脐带 间充质干细胞 蚕丝素 支架 组织工程 

分 类 号：R318.08[医药卫生—生物医学工程]