慢性乙型肝炎病毒感染者外周血自然杀伤细胞免疫效应分子的表达  

Expression profile of immune effector molecules in natural killer cells in patients with chronic hepatitis B

作　　者：李曼[1] 姚嫣[1] 高月求[1] 

机构地区：[1]上海中医药大学附属曙光医院肝肾疾病病证教育部重点实验室,200021

出　　处：《中华肝脏病杂志》2010年第2期96-100,共5页Chinese Journal of Hepatology

基　　金：上海市教育委员会E研究院建设计划项目（03008）;上海市教委曙光计划;上海市科委引导项目（074119608）;上海市浦东新区创新项目

摘　　要：目的研究慢性HBV感染者外周血自然杀伤细胞（NK细胞）免疫效应分子表达与感染状态的相关性。方法健康人作为对照组，慢性HBV感染者根据感染状态分为肝功正常高病毒复制组、肝功能正常低病毒复制组、肝功能异常高病毒复制组、肝功能异常低病毒复制组。应用流式细胞法检测外周血NK细胞的穿孔素（PF）、颗粒酶B（Gr B）、颗粒溶素（GNLY）、肿瘤坏死因子（TNF）α和干扰素（IFN）γ的表达，分析其与感染状态的相关性。组间比较用单因素方差分析；相关性分析采用直线相关Pearson分析。结果阳性细胞百分比同对照组（31．50％±27．64％）相比，肝功能正常高病毒复制组（59．74％±30．82％）和肝功能正常低病毒复制组（61．89％±33．30％）的GNLY表达增加有统计学意义；同对照组（57．38％±23．69％）相比，肝功正常高病毒复制组（39．89％±21．30％）、肝功能异常高病毒复制组（37．54％±18．79％）的IFNγ表达降低，P值均〈0．05，差异有统计学意义；肝功能正常高病毒复制组的PF、Gr B、GNLY（阳性细胞百分比分别为56．98％±38．34％、81．53％±19．58％、59．74％±30．82％）和肝功能正常低病毒复制组的PF、Gr B、GNLY（阳性细胞百分比分别为62．95％±31．98％、84．51％±14．57％、61．89％±33．30％）表达高于肝功能异常高病毒复制组（阳性细胞百分比分别为35．47％±29．64％，66．55％±22．92％，42．03±33．17％），P值均〈0．05，差异有统计学意义；除了TNFα和GNLY不具有相关性外，PF、Gr B、GNLY、TNF仅和IFNY之间呈两两正相关关系。结论高病毒复制水平的HBV感染者外周血NK细胞表达的IFNγ明显低于正常对照组；肝功能正常的HBV感染者外周血NK细胞表达的PF、Gr B、GNLY水平明显高于肝功能异常者。Objective To investigate the expression profile of immune effector molecules in periph- eral natural killer cells （NK） in patients with chronic hepatitis virus B. Methods According to the infection status, patients were divided into four experiment groups： normal hepatic function and high HBV DNA level group, normal hepatic function and low HBV DNA level group, abnormal hepatic function and high HBV DNA level group and abnormal hepatic function and low HBV DNA level group. The expression of perforin （PF）, granzyme B （Gr B）, granulysin （GNLY）, tumor necrosis factor alpha （TNF α ） and interferon gamma （IFN γ ） in NK cells were detected by flow cytometer. Results Compared with control group （31.50% ± 27.64%）, the expression of GNLY was significantly increased in normal hepatic function and high HBV DNA level group （59.74% ± 30.82%） and normal hepatic function and low HBV DNA level group （61.89% ± 33.30%）; the expression of IFN y in normal hepatic function and high HBV DNA level group （39.89% ± 21.30%） and abnormal hepatic function and high HBV DNA level group （37.54% ± 18.79%） was lower than that in normal control group （57.38% ± 23.69%）; the expression of PF, GrB, GNLY in abnormal hepatic function and high HBV DNA level group （35.47% ± 29.64%, 66.55% ± 22.92%, 42.03% ± 33.17%） was lower than that in normal hepatic function and high HBV DNA level group （56.98% ± 38.34%, 81.53% ± 19.58%, 59.74% ± 30.82%） and normal hepatic function and low HBV DNA level groups （62.95% ± 31.98%, 84.51% ± 14.57%, 61.89% ± 33.3%）; there were positive correlations between ef PF, Gr B, GNLY, TNF α, and IFN γ. Conclusion The expression of IFN γ in NK cells from patients with high HBV DNA replication level is lower than that in normal control group; the expression of PF, Gr B and GNLY in NK cells from patients with normal hepatic function is higher than that in NK cells from patients with abnormal hepatic function.

关 键 词：肝炎病毒 乙型 颗粒酶类 干扰素Γ 肿瘤坏死因子α 穿孔素 颗粒溶素 

分 类 号：R51[医药卫生—内科学]