ITS序列鉴定真菌性鼻窦炎病原的方法评价  

Evaluation of ITS region sequencing strategy for the identification of the pathogenic fungi from fungal sinusitis tissues

作　　者：鲁辛辛[1] 耿佳靖[1] 李云川[1] 周兵[1] 袁梁[1] 王向东[1] 张孜[1] 韩德民[1] 

机构地区：[1]首都医科大学附属北京同仁医院检验科,100730

出　　处：《中华检验医学杂志》2010年第2期126-131,共6页Chinese Journal of Laboratory Medicine

基　　金：国家自然科学基金资助项目（30772066）

摘　　要：目的建立ITS（包括ITS1-5．8 rRNA—ITS2）测序鉴定真菌性鼻窦炎病原的方法。方法收集北京同仁医院2006-2008年，经临床与CT诊断为真菌性鼻窦炎，并行鼻内镜手术切除的组织标本270份。所有标本分别进行组织病理检查、压片直接镜检、真菌培养鉴定和核糖体RNA转录间隔区测序分析，通过方法比较，评价序列分析直接鉴定病原真菌的可行性，同时分析真菌性鼻窦炎病原学特征。结果在270份标本中，组织病理阳性率为80．0％（216／270），压片阳性率为80．0％（216／270），真菌培养阳性率为53．0％（143／270），ITS测序阳性率为63．0％（170／270）。经培养得到22个种，6个属。ITS测序鉴定32个种。培养与ITS序列种水平符合率为76．1％（102／143）。结论ITS测序可成为真菌鉴定的辅助工具。Objective To establish a molecular technique of internal transcribed spacer （ITS） sequencing to identify pathogenic fungi species from the fungal sinusitis tissues. Methods Total 270 sinusitis tissues samples were collected by endoscopic surgery from 2006 to 2008. The histopathology, organize spring clip culturation and ITS region （ ITS region region of fungal rRNA, including ITS1-5.8S rRNA-ITS2） sequencing were employed simultaneously. And then to evaluate the ITS sequencing as the tool for identification of pathogenic fungi directly from clinical samples. Results Of the 270 samples, histopathology positive rate was 80.0% （ 216/270 ）, organize spring clip positive rate was 80. 0% （ 216/ 270）, fungal cuhuration positive rate was 53.0% （143/270）, ITS region sequencing positive rate was 63.0% [ （ 134 ＋ 28 ＋ 8 ）/270 ]. There were 22 species and 6 genera identified by fungal culturation, and 32 species identified by ITS region sequencing. Conclusion ITS region sequencing will become a applicable tool in clinical laboratory in future.

关 键 词：鼻窦炎 真菌病 真菌 序列分析 RNA 

分 类 号：R763[医药卫生—耳鼻咽喉科]