祛风通络方对阿霉素肾病大鼠肾小球基底膜HSPG蛋白及mRNA表达的影响  

Effects of Qufengtongluo Recipe on Expressions of HSPG mRNA and Protein in Adriamycin-induced Nephropathy Rats

作　　者：孙万森[1] 任艳芸[1] 赵艳龙[1] 马巧亚[1] 

机构地区：[1]西安交通大学医学院第二附属医院中西医结合科,西安710004

出　　处：《四川大学学报（医学版）》2010年第2期212-217,共6页Journal of Sichuan University(Medical Sciences)

基　　金：国家自然科学基金(批准号30672967)资助

摘　　要：目的观察阿霉素肾病大鼠肾小球基底膜硫酸肝素蛋白多糖(HSPG)蛋白及其mRNA表达及祛风通络方对其表达的影响。方法尾静脉注射阿霉素建立阿霉素肾病大鼠模型,并随机分为A(空白对照,n=32)、B(模型,n=18)、C(祛风通络方,n=18)、D(祛风通络方+激素,n=18)、E(激素,n=18)、F(苯那普利,n=18)组,应用间接免疫荧光染色、RT-PCR分别检测各组大鼠肾小球基底膜HSPG的荧光分布和HSPGmRNA表达的变化。结果造模成功后透射电镜示B组肾小球足突呈部分或弥漫性融合。干预结束后与B组相比,C、E组HSPG的荧光分布呈连续性或斑片状,平均荧光强度明显增强(P<0.01);D、F组HSPG的荧光呈不连续分布或点状分布,平均荧光强度增强(P<0.01)。干预结束后与B组相比,各干预组HSPGmRNA的表达均上调(P<0.01),尤以C、F组明显。相关分析显示24 h尿蛋白定量与HSPG蛋白表达呈明显负相关(r=-0.7353,P<0.001)。结论肾小球基底膜HSPG的表达异常和分布改变是阿霉素肾病大鼠蛋白尿发生发展的重要机制,祛风通络方可能通过改变HSPG的表达和分布而达到治疗蛋白尿的目的。Objective To investigate the expression of HSPG in glomerular base membrane of adriamycin-induced nephropathy（AN） rats,and the effect of Qufengtongluo recipe on HSPG mRNA expression and proteinuria in AN rats.Methods One hundred forty rats were used in this study, including 32 rats in normal control group.AN was induced in the left rats by a single tail intravenous injection of adriamycin.Three weeks later,90 AN rats were randomly divided into five groups： the nephropathy group（B,n=18）,the Qufeng group（C,n=18）,Qufeng and prednisone group（D,n=18）,prednisone group（E,n=18） and benazepri group（F,n=18）.The rats in these five groups were treated with different combination of Qufeng recipe and prednisone.In each group,renal tissue samples were collected at week 3 and 7.The distribution,expression of HSPG was examined by indirect immunofluorescence,and semi-quantity RT-PCRrespectively Results ① In AN rats,the diffuse fusion and effacement of foot processes were observed when model established.② Compared with nephropathy group,the average fluorescence intensity of HSPG dramatically increased in Qufeng group and prednisone group（P〈0.01）,similarly,it also increased in D and F groups（P〈0.01）.③ Compared with nephropathy group, the expression of HSPG mRNA was significantly up-regulated in other groups.（P〈0.01）,especially in C and F groups.There was significant negative correlation between the expression of HSPG and quantity of 24-hour proteinuria.Conclusion The abnormal expression of HSPG and their altered distributions may be an important molecular mechanism that leads to the occurrence and development of proteinuria in AN rats.The effect of Qufengtongluo recipe on nephrotic syndrome might be related to the alteration of HSPG expression and distribution in glomerulus.

关 键 词：阿霉素肾病 蛋白尿 硫酸肝素蛋白多糖 祛风通络方 

分 类 号：R285.5[医药卫生—中药学]