抗氧化剂对Cu^(2+)、HOCl及动脉壁细胞诱导的HDL氧化修饰的作用研究  

Antioxidants Inhibit the Oxidative Modification of High Density Lipoproteins

作　　者：李晓颖[1] 许飞[1] 邢瑞青[1] 李俊葓[1] 刘秉文[1] 傅明德[1] 傅强[1] 

机构地区：[1]四川大学华西基础医学与法医学院生物化学与分子生物学教研室,成都610041

出　　处：《四川大学学报（医学版）》2010年第2期226-230,共5页Journal of Sichuan University(Medical Sciences)

摘　　要：目的探讨抗氧化剂对高密度脂蛋白(HDL)氧化修饰的抑制机制,为预防HDL的氧化修饰提供实验依据。方法将体外新鲜制备的人血浆HDL分别用不同浓度的硫酸铜(CuSO4)、次氯酸(HOCl)处理或与动脉壁细胞共同温育进行氧化修饰,然后分别测定天然未修饰HDL(N-HDL)、氧化修饰HDL(Ox-HDL)的凝胶电泳相对电泳迁移率(REM)、硫代巴比妥酸反应物质(TBARS)、溶血卵磷脂/卵磷脂比率(LPC/PC)的生成及载脂蛋白成分改变。对比相同条件下,加入抗氧化剂叔丁基甲苯(BHT)、Vit E及Vit C后,以上指标的改变。结果BHT、VitE及Vit C均可显著抑制由Cu2+和HOCl诱导的Ox-HDL的REM增加、TBARS的生成、卵磷脂的水解,载脂蛋白AⅠ和AⅡ的改变。动脉壁细胞诱导的Ox-HDL的琼脂糖REM增加、TRARS的生成及卵磷脂水解也可被BHT、Vit E及Vit C显著抑制。但不同抗氧化剂的作用方式各有不同。结论BHT以及Vit E和Vit C能有效抑制体内外HDL的氧化修饰。Objective To study the role and mechanism of antioxidants on inhibiting oxidative modification of high density lipoproteins（HDL）.Methods Freshly prepared human plasma HDL was treated by incubation with copper ion,hyperchlorite or arterial wall cells.Compared to control,the test groups were treated with addition of different concentration of butylhydroxytoluene（BHT）,vitamin C and vitamin E.Then,the relative electrophoretic mobility（REM）,thiobarbituric acid-reactive substances（TBARS）,ratio of lysolecithin to lecithin（LPC/PC）,and lipoprotein moieties were investigated.Results BHT,vitamin C and vitamin E can significantly inhibit the increasing REM,TBARS,LPC/PC ratio and lipoprotein variation that induced by copper ion and hyperchlorite and arterial wall cells.But these antioxidants act on different manner.Conclusion BHT,vitamin C and vitamin E can inhibit the oxidative modification of HDL and hence could be potential nutrients to prevent atherosclerosis.

关 键 词：抗氧化剂 高密度脂蛋白 氧化修饰 

分 类 号：R543.5[医药卫生—心血管疾病]