低密度脂蛋白受体基因PvuⅡ多态性对高糖低脂膳食诱导的健康青年人血脂比值变化的影响  

Effects of PvuⅡ Polymorphism in Low Density Lipoprotein Receptor Gene on Changes of Serum Lipid Ratios Induced by High-carbohydrate/Low-fat Diet in Healthy Youth

作　　者：杜娟[1] 方定志[1] 龚仁蓉[2] 汤慧[1] 黄鑫[1] 李蓉晖[1] 

机构地区：[1]四川大学华西基础医学与法医学院生物化学与分子生物学教研室,成都610041 [2]四川大学华西医院胸心外科,成都610041

出　　处：《四川大学学报（医学版）》2010年第2期239-242,251,共5页Journal of Sichuan University(Medical Sciences)

基　　金：教育部新世纪优秀人才支持计划(项目编号:NCEF04-0863);四川省科技计划(项目编号:2008HH0008)资助

摘　　要：目的探讨低密度脂蛋白受体基因(LDLR)PvuⅡ多态性对健康青年人血脂比值的影响及在高糖低脂(HC/LF)膳食诱导的血脂比值变化中的作用。方法56名〔(22.89±1.80)岁〕健康志愿者,给予7 d平衡膳食和6 d HC/LF膳食。分别于第1 d、第8 d及第14 d取空腹静脉血,测定血脂水平,计算甘油三酯/高密度脂蛋白-胆固醇(TG/HDL-C)、log(TG/HDL-C)、总胆固醇(TC)/HDL-C、低密度脂蛋白-胆固醇(LDL-C)/HDL-C值。分离基因组DNA,聚合酶链反应-限制性酶切法分析LDLR PvuⅡ位点多态性。分析不同基因型间血脂比值差异。结果不同基因型受试者血脂比值的基础值无明显差异。HC/LF膳食前后,无论是在受试人群整体还是男女分组分析,不同基因型之间血脂比值均无明显差异。与HC/LF膳食前相比,在受试人群整体,不同基因型受试者LDL-C/HDL-C和TC/HDL-C均降低(P<0.05)。男女分组分析发现,与HC/LF膳食前相比,HC/LF膳食后TC/HDL-C在男性和女性均下降(P<0.05),但不受LDLR PvuⅡ多态性影响;TG/HDL-C和log(TG/HDL-C)仅在女性升高(P<0.05),也不受LDLR PvuⅡ多态性影响;而LDL-C/HDL-C在女性P1P1基因型、P2携带者和男性P1P1基因型下降(P<0.05)。结论LDLR PvuⅡ位点多态性在健康男性青年与HC/LF膳食诱导的LDL-C/HDL-C改变可能相关联。Objective To investigate the effects of low density lipoprotein receptor gene（LDLR） PvuⅡ polymorphism on serum lipid ratios and their responses to high-carbohydrate/low-fat（HC/LF） diet in healthy youth.Methods After a regular diet for 7 days of wash-out, 56 health youth（（22.89±1.80） years old）） were given LF/HC diet for 6 days.The regular diet contained 54% carbohydrate,15% protein,and 31% fat of the total energy.The LF/HC diet contained 70% carbohydrate,15% protein,and 15% fat of the total energy.The serum lipids were measured on the 1^st,the 8^th and the 14^th days.The ratios of TG/HDL-C,log（TG/HDL-C）,TC/HDL-C,and LDL-C/HDL-C were calculated.The polymorphism of LDLR was detected by PCR-RFLP method and its relationship with serum lipid ratios was analyzed.Results No significant difference was found of the lipid ratios at baseline in subjects with different genotypes in both genders.Before or after HC/LF diet,no significant difference was found of the lipid ratios between the subjects with different genotypes in the whole study population or in males and females separately.Compared with those of before HC/LF diet,all the subjects experienced significant decrease of TC/HDL-C and LDL-C/HDL-C（P〈0.05）.When gender was taken into account,significant increases of TG/HDL-C and log（TG/HDL-C）were found only in females（P〈0.05）.All the subjects experienced significant decrease of TC/HDL-C regardless of gender and genotype（P〈0.05）.As for LDL-C/HDL-C,significant decrease was found only in males with P1P1 and female subjects（P〈0.05）.Conclusion Polymorphism of LDLR PvuⅡ may influence the response of LDL-C/HDL-C to HC/LF diet in males.

关 键 词：低密度脂蛋白受体 基因多态性 高糖低脂膳食 血脂比值 

分 类 号：R589.2[医药卫生—内分泌]