2′,5′-寡腺苷酸合成酶L和干扰素诱导蛋白2基因与系统性红斑狼疮相关性研究  

Interferon-inducible Genes 2′,5′-oligoadenylate Synthetase-like and Tetratricopeptide Repeats 2 Are Correlated with Clinical Features of Patients with Systemic Lupus Erythematosus

作　　者：赵臻怡[1] 杨南萍[1] 黄向阳[1] 李敏[1] 刘潇潇[1] 张杰[1] 叶扬[1] 石连杰[1] 钟雪[1] 

机构地区：[1]四川大学华西医院风湿免疫科,成都610041

出　　处：《四川大学学报（医学版）》2010年第2期247-251,共5页Journal of Sichuan University(Medical Sciences)

摘　　要：目的探讨系统性红斑狼疮(SLE)患者外周血白细胞中干扰素诱导表达2′,5′-寡腺苷酸合成酶L(OASL)和干扰素诱导蛋白2(IFIT2)基因的实时定量表达水平与SLE疾病特异性和疾病活动的相关性。方法收集50例SLE患者,25例非SLE自身免疫性疾病患者,25例健康对照人群的临床资料,取外周血抽提总RNA并逆转录成cDNA,运用SYBR green dyeI实时定量聚合酶链反应(PCR)在Bio-Rad 96孔基因测序仪上检测SLE患者组和非SLE患者组及健康对照组的OASL和IFIT2 mRNA定量表达水平(以ΔCT表示)的差异,并根据各病情不同进行分组比较,以及对其与SLEDAI和临床各相关指标相关性进行评价分析。结果SLE患者总体OASLmRNAΔCT值(4.83±0.41)与非SLE患者(3.26±0.47)差异有统计学意义(P=0.029),与健康对照者(3.07±0.54)差异有统计学意义(P=0.014)。SLE患者总体IFIT2 mRNAΔCT值(2.85±0.41)与非SLE患者(1.19±0.52)差异有统计学意义(P=0.019),与健康对照组(1.07±0.47)差异有统计学意义(P=0.011)。SLE患者总体OASLmRNAΔCT值与SLEDAI有相关性(r=0.324,P=0.022),与免疫球蛋白A(IgA)有相关性(r=0.357,P=0.012),IFIT2 mRNAΔCT值与SLEDAI有相关性(r=0.399,P=0.004),与血白细胞有相关性(r=0.393,P=0.005),与IgA有相关性(r=0.283,P=0.049)。结论OASL和IFIT2在SLE患者中均表达上调,对SLE患者的病情活动度判断有意义,两者在SLE发病中均有一定作用,抑制其过度表达可能成为治疗SLE的新方法。Objective To investigate the expression levels of 2′,5′-oligoadenylate synthetase-like（OASL） and interferon induced protein with tetratricopeptide repeats 2（IFIT2） genes in the peripheral blood leukocyte of patients with systemic lupus erythematosus（SLE）,and to evaluate the relations between these gene expression levels and disease activity.Methods The clinical data of 50 SLE patients,25 non-SLE patients with rheumatic diseases,and 25 normal controls were collected.Specimens of peripheral blood were collected;total RNA was extracted and transcribed into cDNA.SYBR green dye based real-time quantitative PCR method was applied to compare the expression levels（indicated as ΔCT value） of OASL and IFIT2 in patients with SLE and those in the controls.Results The ΔCT value of OASL expression level of the SLE patients was（4.83±0.41）,significantly higher than those of the non-SLE patients（3.26±0.47） and normal controls（3.07±0.54,both P〈0.05）,The ΔCT value of IFIT2 expression level of the SLE patients was（2.85±0.41）,significantly higher than those of the non-SLE patients（1.19±0.52） and normal controls（1.07±0.47,both P〈0.05）.The ΔCT value of OASL is related with the SLEDAI scores and immunoglobulin A.（Both P〈0.05）.The ΔCT value of IFIT2 is related with the SLEDAI scores and IgA,white blood cell（both P〈0.05）.Conclusion The value of IFIT2 and OASL expression level of the SLE patients is up-regulation,the real time expression levels of OASL and IFIT2 genes are associated with SLE disease activity.To inhibit the expression of OASL and IFIT2 may become a novel therapeutic approach for SLE.

关 键 词：系统性红斑狼疮 荧光定量聚合酶链反应 2′ 5′-寡腺苷酸合成酶L 干扰素诱导蛋白2 干扰素 

分 类 号：R593.241[医药卫生—内科学]