依那普利对大鼠慢性支气管肺炎肺组织蛋白组表达变化的影响  

Changes of Proteomics in the Lung of Rats Subjected to Acrolein Inhalation after Enalapril Administration

作　　者：张云辉[1,2] 赵珊[1,2] 王盛兰[1,2] 袁斌[1,2] 王昭君[3] 张丽[3] 黄云超[4] 

机构地区：[1]昆明医学院附属昆华医院 [2]云南省第一人民医院呼吸内科,昆明650000 [3]昆明医学院神经科学研究所,昆明650031 [4]昆明医学院第三附属医院胸心血管外科,昆明650000

出　　处：《四川大学学报（医学版）》2010年第2期273-275,279,共4页Journal of Sichuan University(Medical Sciences)

基　　金：国家自然科学基金(批准号36260125)资助

摘　　要：目的采用蛋白组学方法研究丙烯醛雾化吸入致大鼠慢性支气管肺炎模型中依那普利干预后肺组织蛋白组的表达变化,探讨依那普利干预慢性支气管肺炎的分子机制。方法运用双向电泳和质谱分析技术找出丙烯醛雾化吸入组和依那普利干预组的差异蛋白质。结果在两组凝胶图谱中平均检测到545个蛋白点。依那普利干预组与丙烯醛吸入组比较,发现3个明显差异蛋白。经质谱鉴定,3个差异蛋白分别是甘油醛-3-磷酸脱氢酶,T-激肽原-1-前体,二氢嘧啶酶-2。定量分析结果显示依那普利干预导致肺组织中二氢嘧啶酶-2上调,甘油醛-3-磷酸脱氢酶下调,而T-激肽原-1-前体消失。结论依那普利对大鼠慢性支气管肺炎的干预作用可能与肺组织中这3种蛋白表达变化有关。Objective To study the molecular mechanism of enalapril on rat chronic bronchopneumonia model with proteomics method.Methods Enalapril treatment applied to the recovery of chronic bronchopneumonia in rats,after enalapril administration,two-dimensional electrophoresis and mass spectrometry were performed to analyze the difference of the peptide finger print map and amino acid sequence of rat lung tissues in acrolein inhalation group and enalapril treatment group.Results Three differential protein spots from average 545 protein spots were found in lung tissues of two groups.These protein spots were identified as glyceraldehyde-3-phosphate dehydrogenase,T-kininogen 1 precursor and dihydropyrimidinase-like 2 with mass spectrometry.The significant upregulation in the level of dihydropyrimidinase-like 2,downregulation of glyceraldehyde-3-phosphate dehydrogenase,and the lose of T-kininogen 1 precursor in enalapril treatment group were observed.Conclusion The expression difference in three proteins might contribute to the preventive role of enalapril on acrolein inhalation induced rat chronic bronchopneumonia.

关 键 词：蛋白组学 丙烯醛吸入 依那普利 慢性支气管肺炎 大鼠 

分 类 号：R563.1[医药卫生—呼吸系统]