β-环糊精键合固定相合成方法和色谱性能研究  

Preparation and Evaluation of β-Cyclodextrin Bonded Stationary Phase for High Performance Liquid Chromatography

作　　者：龚银汉[1] 董亚琼[1] 达世禄 王忠华[1] 

机构地区：[1]武汉大学化学系

出　　处：《分析测试学报》1998年第5期5-8,共4页Journal of Instrumental Analysis

基　　金：国家自然科学基金

摘　　要：以两种不同的路线合成β－环瑚精－环氧丙氧丙基键合硅胶固定相（β＿ＣＤ－ＧＰＳ）。比较了两种方法得到的键合相的键合量。采用非水滴定法考察了影响键合反应的因素。选择了合成β＿ＣＤ－ＧＰＳ最佳反应条件，并对其色谱性能进行了考察。β _Cyclodextrin- γ _glycidoxypropylsilyl bonded phase ( β _CD-GPS) for high performance liquid chromatography was synthesized via two different routes:(i) KH_560 reacted on the silica to produce GPS and then β _CD-GPS was obtained by the reaction of β _CD with GPS;(ii)the mixture of the reaction of β _CD with KH_560 reacted on the silica to produce β _CD-GPS.The amounts of bonded β _CD for two stationary phases were calculated according to the results of elemental analysis.The reaction conditions were optimized on the basis of the content of α _epoxy group of γ _glycidoxypropyl bonded silica.The chromatographic performance of β _CD-GPS was studied.

关 键 词：高效液相色谱 环糊精键合 固定相 硅胶 

分 类 号：O657.720.3[理学—分析化学]