生物素的微分脉冲极谱行为及其测定  

Differential Pulse-polarographic Behaviour of Biotin and Its Determination

作　　者：谌英武[1] 陈静[1] 陈启明[1] 李海涛 

机构地区：[1]武汉化工学院精细化工系

出　　处：《分析测试学报》1998年第5期69-71,共3页Journal of Instrumental Analysis

摘　　要：用微分脉冲极谱法研究了生物素的电化学行为，用滴汞电极作工作电极，在０１ｍｏｌ·Ｌ－１ＬｉＣｌ底液中，生物素的峰电位为－１５０Ｖ，生物素浓度在５２８～８７７μｍｏｌ·Ｌ－１范围内与峰高呈线性关系。初步探讨了电极反应机理。该法应用于含生物素样品的分析，可以不经分离，直接测定，结果良好。The electrochemical behaviour of the biotin was examined by differential pulse_polarography with dropping mercury electrode as working electrode.In the solution of 0 1 mol·L -1 LiCl,the peak potential of the biotin was at -1 50 V.The calibration curve was linear over the concentration range of 5 28 ～87 7 μmol·L -1 .The reactive mechanism of electrode was discussed.Biotin in sample of chicken liver was directly determined without separation with satisfactory results.

关 键 词：生物素 脉冲极谱法 测定 

分 类 号：O629.4[理学—有机化学] Q569.03[理学—化学]