TRAIL及其受体DR4、DCR1在多囊卵巢综合征大鼠卵泡发育中的作用  

Role of Tumor Necosis Factor-related Apoptosis-inducing Ligand,Death Receptor 4 and Decoy Receptor 1 during Follicle Development in Polycystic Ovary Syndrome Model Rats

作　　者：张娟[1,2] 朱桂金[1] 王昕荣[1] 胡琳莉[1] 徐蓓[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院生殖中心,武汉430030 [2]中国人民解放军第161中心医院妇产科,武汉430010

出　　处：《生殖与避孕》2010年第2期73-80,共8页Reproduction and Contraception

摘　　要：目的:探讨肿瘤坏死因子相关凋亡诱导配体(TNF-related apoptosis inducing ligand,TRAIL)及其受体——死亡受体4(death receptor 4,DR4)、诱骗受体1(decoy receptor 1,DcR1)在PCOS大鼠卵泡发育中的作用。方法:采用硫酸普拉睾酮钠诱导大鼠PCOS模型,免疫组织化学染色、RT-PCR分析观察TRAIL及其受体DR4、DcR1在PCOS大鼠卵巢颗粒细胞的表达情况。结果:①免疫组织化学结果显示,TRAIL蛋白在PCOS组大鼠卵巢窦状卵泡颗粒细胞的表达较对照组明显增强(P<0.05),在窦前卵泡颗粒细胞的表达两组无显著性差异(P>0.05)。DR4蛋白在两组大鼠窦前卵泡和窦状卵泡的表达无显著性差异(P>0.05)。DcR1蛋白在PCOS组大鼠卵巢窦状卵泡颗粒细胞的表达较对照组明显减弱(P<0.01),在窦前卵泡颗粒细胞的表达两组无显著性差异(P>0.05)。②RT-PCR分析显示,PCOS组大鼠卵巢颗粒细胞TRAIL mRNA的表达较对照组明显增强(P>0.01),DR4mRNA的表达两组无显著性差异(P>0.05),DcR1 mRNA的表达较对照组明显减弱(P<0.01)。结论:TRAIL及其受体DR4、DcR1在PCOS大鼠卵泡发育颗粒细胞凋亡中发挥了调控作用。TRAIL及其受体DR4、DcR1在PCOS颗粒细胞的表达异常可能是导致PCOS卵泡发育障碍的机制之一。Objective：To investigate the role of tumor necosis factor-related apoptosis-inducing ligand（TRAIL）,death receptor 4（DR4） and decoy receptor 1（DcR1） during follicle development in polycystic ovary syndrome model rats.Methods： PCOS rat model induced by codium prasterone sulfate was used to detect the expression of TRAIL,DR 4 and DcR1 in granulosa cells within polycystic ovary syndrome rats through the immunhistochemical staining and reverse transcription polymerase chain reaction（RT-PCR） analysis.Results： 1）Immunohistochemical analysis showed that the expression of TRAIL protein in granulosa cells was significantly stronger in antral follicles from the PCOS rats than that in those from the control rats（P〈0.05）,it was not significantly different in preantral follicles between the two groups（P〉0.05）.The expression of DR4 protein in granulosa cells was not significantly different in preantral and antral follicles between the two groups（P〉0.05）.The expression of DcR1 protein in granulosa cells was significantly weaker in antral follicles from the PCOS rats than that in the control rats（P〈0.01）,it was not significantly different in preantral follicles between the two groups（P〉0.05）.2） RT-PCR analysis showed,the expression of TRAIL mRNA was significantly increased in granulosa cells from thePCOS rats compared with the control（P〈0.01）,the expression of DR4 mRNA was not significantly different ingranulosa cells between the two groups（P〉0.05）,the expression of DcR1 mRNA was significantly decreased in granulosa cells from the PCOS rats than that in the control rats（P〈0.01）.Conclusion： TRAIL,DR4 and DcR1 played a role in regulating the apoptosis of granulosa cells during follicle development in PCOS rats.The abnormal expression of TRAIL,DR4 and DcR1 in granulosa cells may be one of the modulation mechanisms that induced the failure during follicle development in PCOS.

关 键 词：多囊卵巢综合征(PCOS) 大鼠 颗粒细胞 TRAIL 死亡受体4(DR4) 诱骗受体1(DcR1) 

分 类 号：R711.75[医药卫生—妇产科学] R-332[医药卫生—临床医学]