bFGF对促进大鼠移植缺血皮瓣血管化的实验研究  

The study of basic fibroblast growth factor on ischemic vascularization flap in rat

作　　者：黄永军[1] 黄东[1] 牟勇[2] 吴伟炽[1] 张惠茹[1] 葛军委[2] 江钦文[2] 

机构地区：[1]广东省第二人民医院创伤外科,广州510317 [2]广东医学院研究生院

出　　处：《中华显微外科杂志》2010年第1期38-40,共3页Chinese Journal of Microsurgery

摘　　要：目的探讨碱性成纤维细胞生长因子（bFGF）对大鼠移植皮瓣成活作用的影响。方法Wistar大鼠48只，按月龄随机分为A、B、C、D四组，每组12只。制作移植皮瓣模型。A、C组的大鼠多次多点每点注射bFGF60U（0．015m1）；B、D组给予等量生理盐水作为对照。分别于5d、14d观察皮瓣的存活率，分别取相同部位皮瓣组织块行病理组织学检查，并采用计算机图像分析系统和免疫组化方法计算血管密度和血管内皮生长因子（VEGF）阳性血管数。结果大鼠缺血皮瓣血管密度及VEGF阳性表达的血管数A组[（分别为（134．21±4．86）个／mm^2（14．63±2．25）条]多于B组[分别为（118．48±2．31）个／mm^2，（7．45±1．43）条]，C组[分别为（128．67±4．58）个／mm^2，（11．39±1．61）条]多于D组[分别为（115．32±2．18）个／mm^2，（6．96±1．35）条]，差异均有统计学意义（P〈0．05）；A组和C组比较，差异无统计学意义（P〉0．05）。结论在不同月龄大鼠移植皮瓣内注射bFGF蛋白可促进皮瓣血管新生，改善移植皮瓣缺血状态，提高皮瓣成活率。Objective To investigate the application valve of bFGF to improve the viability of transplantation flap in Wistar rat. Methods Forty-eight Wistar rats were randomly divided into two groups based on the age： group A,B（1.5 month old, every group was 12 rats） and group C,D （3 month old, every group was 12 rats）. After an isehemic model completed, recombinant bovine basic fibroblast growth factor （rb-bFGF） was given to groups A and C in ischemie zone by vascularization flap injection and the equality of normal saline to groups B and D. 14 days postoperatively, the muscular tissue was sent for histology, the blood vessel density was calculated by image analysis, and positive VEGF was detected by immunohistochem- istry. Results The member of capillaries and positive VEGF were more in group A than that in B, and also were morein group C than D（P 〈 0.05）, but there were not statistic difference between group A and C （P 〉 0.05）. Conclusion Recombinant bovine bFGF can stimulate angiogenesis and improve the ischemic vascularization flap of rat, which is not associated with their age.

关 键 词：碱性成纤维细胞生长因子 新生血管化 皮瓣 移植 

分 类 号：R622[医药卫生—整形外科]