风湿性心脏病慢性心房颤动患者界嵴连接蛋白40蛋白和基因转录表达  

Protein and mRNA expression of connexin 40 in crista terminalis of patients suffered from rheumatic heart disease with chronic atrial fibrillation

作　　者：赵峰[1] 周勇[1] 李莉[2] 姚均迪[1] 蒋逸风[1] 徐志云[2] 

机构地区：[1]上海解放军第411医院心内科,200081 [2]上海第二军医大学长海医院胸心外科

出　　处：《中华心律失常学杂志》2010年第1期71-74,共4页Chinese Journal of Cardiac Arrhythmias

基　　金：国家自然科学基金资助（30070749）

摘　　要：目的通过检测风湿性心脏病（风心病）慢性心房颤动（房颤）患者界嵴连接蛋白（connexin，Cx）40的蛋白表达、mRNA表达情况，探讨Cx40重构的形式与可能机制。方法选择风心病二尖瓣病变手术患者20例分为窦性心律（窦律）组10例，心房颤动（房颤）组10例，另取6例非风心病窦律患者作为对照组。换瓣术中剪取界嵴组织约200mg。采用Western blot蛋白印迹法检测Cx40蛋白表达量；实时荧光定量聚合酶链反应法检测Cx40mRNA表达水平。结果（1）风心病慢性房颤组界嵴Cx40蛋白表达水平明显较窦律组和对照组低。（2）3组患者界嵴Cx40mRNA表达量差异无统计学意义。结论风心病慢性房颤患者Cx40重构形式是Cx40表达下词；而Cx40重构的具体机制在于转录后水平。Objective To investigate the protein expression and mRNA expression of Cx40 in crista terminalis of patients suffered from rheumatic heart disease with chronic atrial fibrillation. And evaluate the pattern and the mechanism of the remodeling of Cx40. Methods A small piece of myocardial specimen was acquired from crista terminalis during the operation in 20 patients who underwent operation therapy for rheumatic mitral valve disease and 6 patients underwent cardiac surgery for other diseases served as control group. Western blot was used to detect expression of Cx40. Cx40 mRNA expression was detected by real-time fluorescence quantitative PCR method. Results Compared with sinus rhythm,Cx40 expression was decreased in chronic at- rial fibrillation. But the difference of Cx40 mRNA expression among the three groups had no statistical significance. Conclusion The pattern of the Cx40 remodeling in patients suffered from rheumatic heart disease with chronic atrial fibrillation was decline. And the mechanism of the Cx40 remodeling remains in the level after gene transcription of Cx40.

关 键 词：风湿性心脏病 连接蛋白40 心房颤动 界嵴 

分 类 号：R541.2[医药卫生—心血管疾病] R541.75[医药卫生—内科学]