多药耐药鲍氏不动杆菌可移动遗传元件研究  

Mobile Genetic Elements in Multidrug-resistant Acinetobacter baumannii

作　　者：朱健铭 马兆龙[2] 姜如金[1] 吴康乐[1] 

机构地区：[1]杭州市余杭区中医院检验科,浙江杭州311106 [2]杭州市余杭区中医院骨伤科,浙江杭州311106

出　　处：《中华医院感染学杂志》2010年第6期755-758,共4页Chinese Journal of Nosocomiology

基　　金：杭州市科技发展计划项目(20090833B35);杭州市卫生科技计划项目(2008B0113);杭州市余杭区重大科技项目(2008001)

摘　　要：目的了解临床分离的多药耐药鲍氏不动杆菌(MDR-ABA)可移动遗传元件的存在状况。方法收集住院患者中分离的62株MDR-ABA,采用聚合酶链反应(PCR)及序列分析的方法检测接合性质粒遗传标记(traA、trbC),插入序列(ISaba1、IS1133、ISEcp1),转座子遗传标记(merA、tnsA),整合子遗传标记(intⅠ1、intⅠ2、intⅠ3)等可移动遗传元件,并将先前报道的转座子遗传标记新型转座酶基因tnpU、tnp513合并总结分析。结果62株MDR-ABA中,4种可移动遗传元件基因ISaba1、tnpU、tnp513和intⅠ1阳性株数分别为55株(88.7%)、34株(54.8%)、35株(56.5%)和34株(54.8%),其余8种基因均阴性;55株(88.7%)至少检出1种基因,33株(53.2%)同时4种基因阳性。结论MDR-ABA存在严重的β-内酰胺类、氨基糖苷类、喹诺酮类、四环素和复方新诺明等多药耐药状况可能与ISaba1、tnpU、tnp513和intⅠ1等可移动遗传元件标记携带率高有关,在MDR-ABA中检出ISaba1、tnpU、tnp513等3种基因插入序列和转座酶基因为国内首次。OBJECTIVE To investigate the distribution of mobile genetic elements in multidrug-resistant Acinetobacter baumannii（MDR-ABA） isolated from clinical samples.METHODS Sixty-two strains of MDR-ABA were isolated from inpatients.Then,10 kinds of mobile genetic elements： genetic markers of conjugal plasmids（traA and trbC）,insertion sequences（ISaba1,IS1133 and ISEcp1）,genetic markers of transposons（merA and tnsA）,and genetic markers of integrons（intⅠ1,intⅠ2 and intⅠ3） were analyzed by PCR and verified by DNA sequencing.Combining with genetic markers of transposons（tnpU and tnp513） reported before,all data were analyzed as a whole.RESULTS In 62 strains of MDR-ABA,55 strains（88.7%）,34（54.8%）,35（56.5%）and 34（54.8%） were detected to carry ISaba1,tnpU,tnp513 and intⅠ1,respectively.However,the rest 8 kinds of genes couldn′t be detected out.Furthermore,55 strains（88.7%）were detected out to carry at least 1 kind of gene.In addition,33 strains（53.2%）were detected to carry 4 kinds of genes together.CONCLUSIONS The group of MDR-ABA shows multidrug resistance to beta-lactams,aminoglycosides,quinolones,tetracycline and trimethoprim-sulfamethoxazole antimicrobial agents might result from high rate of mobile genetic elements,such as ISaba1,tnpU,tnp513 and intⅠ1,harboring on strains.It′s the first report that insertion sequence ISaba1,and genetic markers of transposons tnpU and tnp513 are detected in MDR-ABA in China.

关 键 词：鲍氏不动杆菌 多药耐药 可移动遗传元件 基因 

分 类 号：R378[医药卫生—病原生物学]