姜黄素抑制子宫内膜异位症血管形成的实验研究  

Inhibition effect of curcumin on angiogenesis in ectopic endometrium of rats

作　　者：张颖[1] 曹洪[2] 彭海英[1] 江胜芳[1] 胡玥玥[1] 黄晓卉[1] 张昌军[1] 

机构地区：[1]郧阳医学院附属人民医院生殖中心,湖北十堰442000 [2]郧阳医学院附属人民医院骨关节外科,湖北十堰442000

出　　处：《中国医院药学杂志》2010年第5期363-366,共4页Chinese Journal of Hospital Pharmacy

基　　金：湖北省教育厅科学研究项目(编号:B20092405)

摘　　要：目的:观察姜黄素对大鼠子宫内膜异位症(endometriosis,EMs)局部血管形成的影响和抗血管形成作用。方法:制作Wistar大鼠EMs动物模型,4周后将大鼠分为正常组、假手术组、模型组、姜黄素低剂量组及姜黄素高剂量组5组,持续用药28d,SP免疫组化法测定在位内膜和异位组织灶中微血管密度(microvessel density,MVD)的表达,western blotting测定局部血管内皮生长因子(vascular endothelial growth factor,VEGF)蛋白量的表达。结果:姜黄素治疗模型大鼠EMs,4周后异位组织中MVD和VEGF蛋白量的表达明显低于模型组,差异有显著性(P<0.05),而在位内膜中MVD的表达与模型组相比,差异无显著性(P>0.05);姜黄素可剂量依赖性的抑制EMs异位组织的增生。结论:姜黄素对子宫内膜异位症大鼠异位组织中MVD的表达和VEGF蛋白的表达均有抑制作用,作用呈剂量依赖性。姜黄素有望成为子宫内膜异位症抗血管形成治疗的新药。OBJECTIVE To observe the effect of curcumin on microvessel density（MVD） and antiangiogenesis in ectopic endometrium of rats. METHODS Wistar rats endometriosis models were established, after 4 weeks the model rats were divided into the control group, sham operated group,model group,high-dose group and low-dose group after 4 weeks. MVD was ob served by immunohistochemistry （SP technique） and the protein expression level of vascular endothelial growth factor（VEGF） was detected by Western blotting in eutopic and ectopic endometrium after 28 days treatment. RESULTS MVD and the protein expression level of VEGF in ectopic endometrium were decreased remarkably after treatment of eurcumin after 4 weeks（P〈0. 05）, while the difference was not significant in the eutopic endometriunx Curcumin could significantly inhibit proliferation of ectopic endometrium in a dose-dependent manner. CONCLUSION Curcumin has the effect on inhibiting the MVD and anglogenesis in ectopic endometrium of rats, which effects are dose-dependent. Cuecumin maybe a new drug of antiangiogenesis to the endometriosis.

关 键 词：姜黄素 子宫内膜异位症 微血管密度 血管内皮生长因子 血管形成 

分 类 号：R969[医药卫生—药理学]