血三七活性部位特征图谱  

Studies on fingerprints of the active site of polygonum amplexicaule

作　　者：向梅先[1,2] 杜小青[2] 徐宗[2] 闫云君[1] 

机构地区：[1]华中科技大学生命科学与技术学院,湖北武汉430075 [2]中南民族大学药学院,湖北武汉430074

出　　处：《中国医院药学杂志》2010年第5期366-369,共4页Chinese Journal of Hospital Pharmacy

基　　金：国863计划重点项目课题(编号:2006AA020203);教育部新世纪优秀人才基金(编号:NCET-07-0336)资助;武汉市晨光项目(SYZ09005)

摘　　要：目的:建立血三七药材特征图谱,为科学评价血三七质量提供依据。方法:采用高效液相色谱技术,选择Diamonsil TM C18柱(250mm×4.6mm,5μm),以甲醇-0.2%磷酸为流动相梯度洗脱,流速1mL·min-l,检测波长347nm,柱温为室温。结果:建立了9个不同产地血三七黄酮类成分的指纹图谱,发现了11个共有峰,确定了其相对保留时间和相对峰面积的比值范围,每个产地血三七指纹图谱相似度均在90%以上,但也存在一定差异。结论:利用该特征图谱,可以对不同产地血三七药材进行质量控制。OBJECTIVE To set up fingerprints of Polygonum amplexicaule var. sinense from different production areas in order to provide an academic basis for the scientific evaluation of the quality of P. amplexicaule vat. sinense. METHODS High-performance liquid chromatography technology was used, selected Diamonsil TM C18 column （250 mm × 4. 6 mm, 5 μm）, and used different dense methanol： 0. 2% phosphoric acid as the mobile phase gradient elution, the flow rate was 1 mL-min^-1 , the detection wavelength was 347 nm, the column temperature was set at room temperature. RESULTS The fingerprints were set up for the flavonoids of P. amplexicaule var. sinense from nine different production places, a total of 11 peaks were found, and their relative retention time and relative peak area ratio of the scope were determined. Each fingerprint of P. amplelicaule from different places exhibited 90% of similarity. CONCLUSION These fingerprint atlas may be used as a standard for the quality control of P. arnplexricaule vat. sinense raw materials from different regions.

关 键 词：血三七 高效液相色谱图 特征图谱 活性部位 黄酮类成分 

分 类 号：R927.2[医药卫生—药学]